Strategic feeding of ammonium and metal ions for enhanced GLA-rich lipid accumulation in Cunninghamella bainieri 2a1

Shuwahida Shuib, Wan Nazatul Naziah Wan Nawi, Ekhlass M. Taha, Othman Omar, Abdul Jalil Abdul Kader, Mohd. Sahaid Kalil, Aidil Abdul Hamid

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Strategic feeding of ammonium and metal ions (Mg2+, Mn 2+, Fe3+, Cu2+, Ca2+, Co 2+, and Zn2+) for enhanced GLA-rich lipid accumulation in C. bainieri 2A1 was established. When cultivated in nitrogen-limited medium, the fungus produced up to 30% lipid (g/g biomass) with 12.9% (g/g lipid) GLA. However, the accumulation of lipid stopped at 48 hours of cultivation although glucose was abundant. This event occurred in parallel to the diminishing activity of malic enzyme (ME), fatty acid synthase (FAS), and ATP citrate lyase (ACL) as well as the depletion of metal ions in the medium. Reinstatement of the enzymes activities was achieved by feeding of ammonium tartrate, but no increment in the lipid content was observed. However, increment in lipid content from 32% to 50% (g/g biomass) with 13.2% GLA was achieved when simultaneous feeding of ammonium, glucose, and metal ions was carried out. This showed that the cessation of lipid accumulation was caused by diminishing activities of the enzymes as well as depletion of the metal ions in the medium. Therefore, strategic feeding of ammonium and metal ions successfully reinstated enzymes activities and enhanced GLA-rich lipid accumulation in C. bainieri 2A1.

Original languageEnglish
Article number173574
JournalScientific World Journal
Volume2014
DOIs
Publication statusPublished - 2014

Fingerprint

Cunninghamella
Ammonium Compounds
Metal ions
ammonium
lipid
Metals
Ions
Lipids
ion
metal
Enzyme activity
Enzymes
Biomass
enzyme activity
glucose
ATP Citrate (pro-S)-Lyase
enzyme
Glucose
Fatty Acid Synthases
biomass

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Strategic feeding of ammonium and metal ions for enhanced GLA-rich lipid accumulation in Cunninghamella bainieri 2a1. / Shuib, Shuwahida; Wan Nawi, Wan Nazatul Naziah; Taha, Ekhlass M.; Omar, Othman; Abdul Kader, Abdul Jalil; Kalil, Mohd. Sahaid; Abdul Hamid, Aidil.

In: Scientific World Journal, Vol. 2014, 173574, 2014.

Research output: Contribution to journalArticle

Shuib, Shuwahida ; Wan Nawi, Wan Nazatul Naziah ; Taha, Ekhlass M. ; Omar, Othman ; Abdul Kader, Abdul Jalil ; Kalil, Mohd. Sahaid ; Abdul Hamid, Aidil. / Strategic feeding of ammonium and metal ions for enhanced GLA-rich lipid accumulation in Cunninghamella bainieri 2a1. In: Scientific World Journal. 2014 ; Vol. 2014.
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