Kesan Penyimpanan Sampel Air Liur Terhadap Kualiti DNA Genom

Translated title of the contribution: Storage effect of air liur sample on quality of genomic DNA

Rohaya Megat Abdul Wahab, Farah Amirah Mohd Nasri, Intan Zarina Zainol Abidin, Zaidah Zainal Ariffin, Muhammad Dain Yazid, Shahrul Hisham Zainal Ariffin

Research output: Contribution to journalArticle

Abstract

Air liur is a potential source of DNA easily obtained for clinical studies because it is non-invasive compared to blood samples. This study was carried out to isolate and purify genomic DNA from human saliva sample and to study storage effect on the quality of genomic DNA. Air liur samples (n=5) were kept in Tris-NaCl EDTA (TNE) buffer at room temperature (25°C) according to fixed time period which are; fresh (without storage), 1, 2, 3 and 4 months. Isolation and purification of DNA was carried out using phenol-chloroform method. Next, PCR was conducted to determine the purity of extracted DNA by using amplification of beta-globin sequence region and identify bacterial existence using the sequence that codes for 16S rDNA. Only human beta-globin genomic DNA fragment was successfully amplified from fresh sample. Air liur sample kept in TNE buffer at room temperature was not able to stabilize human genomic DNA at long term and worked for short term storage which was less than a month. In conclusion, fresh saliva sample is needed to isolate genomic DNA.

Original languageMalay
Pages (from-to)909-915
Number of pages7
JournalSains Malaysiana
Volume46
Issue number6
DOIs
Publication statusPublished - 1 Jun 2017

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Air
DNA
beta-Globins
Saliva
Edetic Acid
Buffers
Temperature
Chloroform
Ribosomal DNA
Phenol
Polymerase Chain Reaction

ASJC Scopus subject areas

  • General

Cite this

Kesan Penyimpanan Sampel Air Liur Terhadap Kualiti DNA Genom. / Megat Abdul Wahab, Rohaya; Nasri, Farah Amirah Mohd; Abidin, Intan Zarina Zainol; Ariffin, Zaidah Zainal; Yazid, Muhammad Dain; Zainal Ariffin, Shahrul Hisham.

In: Sains Malaysiana, Vol. 46, No. 6, 01.06.2017, p. 909-915.

Research output: Contribution to journalArticle

Megat Abdul Wahab, Rohaya ; Nasri, Farah Amirah Mohd ; Abidin, Intan Zarina Zainol ; Ariffin, Zaidah Zainal ; Yazid, Muhammad Dain ; Zainal Ariffin, Shahrul Hisham. / Kesan Penyimpanan Sampel Air Liur Terhadap Kualiti DNA Genom. In: Sains Malaysiana. 2017 ; Vol. 46, No. 6. pp. 909-915.
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