Stemness gene expression profile of human adipose derived stem cells in long-term culture.

W. S Wan Kamarul Zaman, Suzana Makpol, S. Santhapan, Chua Kien Hui

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

It is crucial to know whether stem cells retain its stemnness properties after advance in vitro manipulation. The objective of this study was to investigate the stemness gene expression of human adipose tissue derived stem cells (ADSCs) in long-term culture using quantitative RT-PCR technique. Our data showed that the expression level of Sox-2, Rex-1, FGF-4, Nanog, Nestin, BST-1, FZD-9 and Oct-4 were decreased gradually in long-term culture. This could mean that the ability of ADSCs to differentiate into other cell lineages reduce after extensive culture.

Original languageEnglish
Pages (from-to)61-62
Number of pages2
JournalThe Medical journal of Malaysia
Volume63 Suppl A
Publication statusPublished - Jul 2008

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Transcriptome
Stem Cells
Adipose Tissue
Fibroblast Growth Factor 1
Nestin
Cell Lineage
Gene Expression
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Stemness gene expression profile of human adipose derived stem cells in long-term culture. / Zaman, W. S Wan Kamarul; Makpol, Suzana; Santhapan, S.; Kien Hui, Chua.

In: The Medical journal of Malaysia, Vol. 63 Suppl A, 07.2008, p. 61-62.

Research output: Contribution to journalArticle

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