Stage-dependent effects of Quassia borneensis Noot. (Simaroubaceae) chloroform extracts on erythrocytes infected with chloroquine-resistant plasmodium berghei NK65

Wan Marahaini Wan Razali, Julenah Ag Nuddin, Shafariatul Akmar Shak, Ahmad Rohi Ghazali

Research output: Contribution to journalArticle

Abstract

This study was conducted to screen the antiplasmodial activity of chloroform extracts of Quassia borneensis against different intraerythrocytic cycle of chloroquine-resistant Plasmodium berghei NK65. The antiplasmodial activity was measured using SYBR green I assay for determining the concentration that inhibited 50% (IC50) of the Plasmodium’s activity after 24 hours of treatment (0.0001-100 mg mL-1). All of the extracts showed potent activity against all of the cycles. The trophozoite and schizont stages were considerably more sensitive to the extracts than the ring-stages parasites. The values were compared to the controls treated with chloroquine and artemisinin. Hence, the chloroform extracts of roots and bark of Q. borneensis demonstrated potent activities towards each life cycle of chloroquine-resistant P. berghei and has a potential to be developed as an antimalarial agent.

Original languageEnglish
Pages (from-to)13-17
Number of pages5
JournalJurnal Teknologi
Volume77
Issue number3
DOIs
Publication statusPublished - 2015

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Chlorine compounds
Life cycle
Assays
Parasites

Keywords

  • Antimalarial
  • Intraerythrocytes
  • P. berghei
  • Q. borneensis

ASJC Scopus subject areas

  • Engineering(all)

Cite this

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title = "Stage-dependent effects of Quassia borneensis Noot. (Simaroubaceae) chloroform extracts on erythrocytes infected with chloroquine-resistant plasmodium berghei NK65",
abstract = "This study was conducted to screen the antiplasmodial activity of chloroform extracts of Quassia borneensis against different intraerythrocytic cycle of chloroquine-resistant Plasmodium berghei NK65. The antiplasmodial activity was measured using SYBR green I assay for determining the concentration that inhibited 50{\%} (IC50) of the Plasmodium’s activity after 24 hours of treatment (0.0001-100 mg mL-1). All of the extracts showed potent activity against all of the cycles. The trophozoite and schizont stages were considerably more sensitive to the extracts than the ring-stages parasites. The values were compared to the controls treated with chloroquine and artemisinin. Hence, the chloroform extracts of roots and bark of Q. borneensis demonstrated potent activities towards each life cycle of chloroquine-resistant P. berghei and has a potential to be developed as an antimalarial agent.",
keywords = "Antimalarial, Intraerythrocytes, P. berghei, Q. borneensis",
author = "{Wan Razali}, {Wan Marahaini} and Nuddin, {Julenah Ag} and Shak, {Shafariatul Akmar} and Ghazali, {Ahmad Rohi}",
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language = "English",
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AU - Wan Razali, Wan Marahaini

AU - Nuddin, Julenah Ag

AU - Shak, Shafariatul Akmar

AU - Ghazali, Ahmad Rohi

PY - 2015

Y1 - 2015

N2 - This study was conducted to screen the antiplasmodial activity of chloroform extracts of Quassia borneensis against different intraerythrocytic cycle of chloroquine-resistant Plasmodium berghei NK65. The antiplasmodial activity was measured using SYBR green I assay for determining the concentration that inhibited 50% (IC50) of the Plasmodium’s activity after 24 hours of treatment (0.0001-100 mg mL-1). All of the extracts showed potent activity against all of the cycles. The trophozoite and schizont stages were considerably more sensitive to the extracts than the ring-stages parasites. The values were compared to the controls treated with chloroquine and artemisinin. Hence, the chloroform extracts of roots and bark of Q. borneensis demonstrated potent activities towards each life cycle of chloroquine-resistant P. berghei and has a potential to be developed as an antimalarial agent.

AB - This study was conducted to screen the antiplasmodial activity of chloroform extracts of Quassia borneensis against different intraerythrocytic cycle of chloroquine-resistant Plasmodium berghei NK65. The antiplasmodial activity was measured using SYBR green I assay for determining the concentration that inhibited 50% (IC50) of the Plasmodium’s activity after 24 hours of treatment (0.0001-100 mg mL-1). All of the extracts showed potent activity against all of the cycles. The trophozoite and schizont stages were considerably more sensitive to the extracts than the ring-stages parasites. The values were compared to the controls treated with chloroquine and artemisinin. Hence, the chloroform extracts of roots and bark of Q. borneensis demonstrated potent activities towards each life cycle of chloroquine-resistant P. berghei and has a potential to be developed as an antimalarial agent.

KW - Antimalarial

KW - Intraerythrocytes

KW - P. berghei

KW - Q. borneensis

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