Stacked films immobilization of MBTH in nafion/sol-gel silicate and horseradish peroxidase in chitosan for the determination of phenolic compounds

Jaafar Abdullah, Musa Ahmad, Yook Heng Lee, Nadarajah Karuppiah, Hamidah Sidek

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The stacked-film immobilization of 3-methyl-2-benzothiazolinone hydrazone (MBTH) in hybrid nafion/sol-gel silicate film and horseradish peroxidase (HRP) in chitosan, performed in order to allow the determination of phenolic compounds, was investigated via an optical method. The stacked films were deposited onto a microscope glass slide by a spin-coating technique. The quinone or free radical product formed by the enzymatic reactions of phenolic compounds interacts with MBTH to form azo-dye products, which can be measured spectrophotometrically at a wavelength of 500 nm. The color intensity of the product was found to increase in proportion to the phenolic concentration after 5 min of exposure. The response of the biosensor was linear over concentration ranges of 0.025-0.500, 0.010-0.070 and 0.050-0.300 mM for guaiacol, resorcinol and o-cresol, respectively, and gave detection limits of 0.010, 0.005 and 0.012 mM. The sensor exhibited good sensitivity and stability for at least two months.

Original languageEnglish
Pages (from-to)1285-1292
Number of pages8
JournalAnalytical and Bioanalytical Chemistry
Volume386
Issue number5
DOIs
Publication statusPublished - Nov 2006

Fingerprint

Silicates
Chitosan
Polymethyl Methacrylate
Horseradish Peroxidase
Immobilization
Sol-gels
Azo Compounds
Gels
Guaiacol
Biosensing Techniques
Free Radicals
Glass
Limit of Detection
Coating techniques
Color
Spin coating
Biosensors
Microscopes
Wavelength
Sensors

Keywords

  • Horseradish peroxidase (HRP)
  • Hybrid nafion/sol-gel silicate
  • Optical biosensor
  • Phenolic compounds

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Stacked films immobilization of MBTH in nafion/sol-gel silicate and horseradish peroxidase in chitosan for the determination of phenolic compounds. / Abdullah, Jaafar; Ahmad, Musa; Lee, Yook Heng; Karuppiah, Nadarajah; Sidek, Hamidah.

In: Analytical and Bioanalytical Chemistry, Vol. 386, No. 5, 11.2006, p. 1285-1292.

Research output: Contribution to journalArticle

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