Stable Isotope Markers Differentiate between Mass-Reared and Wild Lepidoptera in Sterile Insect Technique Programs

Rebecca Hood-Nowotny, Ally Harari, Rakesh K. Seth, Wee Suk Ling, Des E. Conlong, David M. Suckling, Bill Woods, Kaouthar Lebdi-Grissa, Gregory Simmons, James E. Carpenter

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this study we identified a number of moth (Lepidoptera) species that are potential targets for the sterile insect technique (SIT), and we assessed the feasibility of using stable isotope signatures as markers to distinguish mass-reared from wild moth species. Large natural differences in the isotopic signatures of commercially available sugars render them novel markers for mass-reared insects. Sugar beet (Beta vulgaris L.; Caryophyllales: Amaranthaceae), a C3 plant, has a stable isotopic signature (a measure of the ratio of the stable isotopes 13C:12C) of around -27‰ relative to Vienna Pee Dee Belemnite (VPDB; the international C isotope standard for the stable isotopes, 13C and 12C), and sugarcane (Saccharum spp.; Poales: Poaceae), a C4 plant, has an isotopic signature of around -11‰. Thus by means of such a distinct isotope ratio in the sugar in the diet, mass-reared insects can be easily distinguished from wild insects with a high degree of certainty. It was shown that the method could be extended using a multiple isotope approach, with 15N or a full suite of C, N, S and O isotopes. Intrinsic isotope marking of mass-reared moths proved to be an accurate means of distinguishing wild from mass-reared populations, based on isotopic differences between the wild host plant species and the diets used in mass-rearing, which where possible, had been manipulated to contain the isotopically divergent sugar type. This intrinsic labeling using stable isotopes could be useful in the assessment of the quality of mass-reared moths, because a stable isotope is a marker that does not affect the insect in any detrimental manner.

Original languageEnglish
Pages (from-to)166-176
Number of pages11
JournalFlorida Entomologist
Volume99
DOIs
Publication statusPublished - 1 Jun 2016

Fingerprint

sterile insect technique
stable isotopes
stable isotope
Lepidoptera
insect
isotopes
moths
moth
isotope
insects
sugar
sugars
Poales
Caryophyllales
mass rearing
diet
Saccharum
Amaranthaceae
belemnite
C4 plants

Keywords

  • Elemental analysis-isotope ratio mass-spectrometry (EA-IRMS)
  • Isotopic signature
  • Larval diet
  • Markers
  • Moths
  • Release/recapture
  • Sterile insects
  • Sugar

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

Cite this

Stable Isotope Markers Differentiate between Mass-Reared and Wild Lepidoptera in Sterile Insect Technique Programs. / Hood-Nowotny, Rebecca; Harari, Ally; Seth, Rakesh K.; Suk Ling, Wee; Conlong, Des E.; Suckling, David M.; Woods, Bill; Lebdi-Grissa, Kaouthar; Simmons, Gregory; Carpenter, James E.

In: Florida Entomologist, Vol. 99, 01.06.2016, p. 166-176.

Research output: Contribution to journalArticle

Hood-Nowotny, R, Harari, A, Seth, RK, Suk Ling, W, Conlong, DE, Suckling, DM, Woods, B, Lebdi-Grissa, K, Simmons, G & Carpenter, JE 2016, 'Stable Isotope Markers Differentiate between Mass-Reared and Wild Lepidoptera in Sterile Insect Technique Programs', Florida Entomologist, vol. 99, pp. 166-176. https://doi.org/10.1653/024.099.sp120
Hood-Nowotny, Rebecca ; Harari, Ally ; Seth, Rakesh K. ; Suk Ling, Wee ; Conlong, Des E. ; Suckling, David M. ; Woods, Bill ; Lebdi-Grissa, Kaouthar ; Simmons, Gregory ; Carpenter, James E. / Stable Isotope Markers Differentiate between Mass-Reared and Wild Lepidoptera in Sterile Insect Technique Programs. In: Florida Entomologist. 2016 ; Vol. 99. pp. 166-176.
@article{e55e9b67590b410abebb14de7a37fb30,
title = "Stable Isotope Markers Differentiate between Mass-Reared and Wild Lepidoptera in Sterile Insect Technique Programs",
abstract = "In this study we identified a number of moth (Lepidoptera) species that are potential targets for the sterile insect technique (SIT), and we assessed the feasibility of using stable isotope signatures as markers to distinguish mass-reared from wild moth species. Large natural differences in the isotopic signatures of commercially available sugars render them novel markers for mass-reared insects. Sugar beet (Beta vulgaris L.; Caryophyllales: Amaranthaceae), a C3 plant, has a stable isotopic signature (a measure of the ratio of the stable isotopes 13C:12C) of around -27‰ relative to Vienna Pee Dee Belemnite (VPDB; the international C isotope standard for the stable isotopes, 13C and 12C), and sugarcane (Saccharum spp.; Poales: Poaceae), a C4 plant, has an isotopic signature of around -11‰. Thus by means of such a distinct isotope ratio in the sugar in the diet, mass-reared insects can be easily distinguished from wild insects with a high degree of certainty. It was shown that the method could be extended using a multiple isotope approach, with 15N or a full suite of C, N, S and O isotopes. Intrinsic isotope marking of mass-reared moths proved to be an accurate means of distinguishing wild from mass-reared populations, based on isotopic differences between the wild host plant species and the diets used in mass-rearing, which where possible, had been manipulated to contain the isotopically divergent sugar type. This intrinsic labeling using stable isotopes could be useful in the assessment of the quality of mass-reared moths, because a stable isotope is a marker that does not affect the insect in any detrimental manner.",
keywords = "Elemental analysis-isotope ratio mass-spectrometry (EA-IRMS), Isotopic signature, Larval diet, Markers, Moths, Release/recapture, Sterile insects, Sugar",
author = "Rebecca Hood-Nowotny and Ally Harari and Seth, {Rakesh K.} and {Suk Ling}, Wee and Conlong, {Des E.} and Suckling, {David M.} and Bill Woods and Kaouthar Lebdi-Grissa and Gregory Simmons and Carpenter, {James E.}",
year = "2016",
month = "6",
day = "1",
doi = "10.1653/024.099.sp120",
language = "English",
volume = "99",
pages = "166--176",
journal = "Florida Entomologist",
issn = "0015-4040",
publisher = "Florida Entomological Society",

}

TY - JOUR

T1 - Stable Isotope Markers Differentiate between Mass-Reared and Wild Lepidoptera in Sterile Insect Technique Programs

AU - Hood-Nowotny, Rebecca

AU - Harari, Ally

AU - Seth, Rakesh K.

AU - Suk Ling, Wee

AU - Conlong, Des E.

AU - Suckling, David M.

AU - Woods, Bill

AU - Lebdi-Grissa, Kaouthar

AU - Simmons, Gregory

AU - Carpenter, James E.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - In this study we identified a number of moth (Lepidoptera) species that are potential targets for the sterile insect technique (SIT), and we assessed the feasibility of using stable isotope signatures as markers to distinguish mass-reared from wild moth species. Large natural differences in the isotopic signatures of commercially available sugars render them novel markers for mass-reared insects. Sugar beet (Beta vulgaris L.; Caryophyllales: Amaranthaceae), a C3 plant, has a stable isotopic signature (a measure of the ratio of the stable isotopes 13C:12C) of around -27‰ relative to Vienna Pee Dee Belemnite (VPDB; the international C isotope standard for the stable isotopes, 13C and 12C), and sugarcane (Saccharum spp.; Poales: Poaceae), a C4 plant, has an isotopic signature of around -11‰. Thus by means of such a distinct isotope ratio in the sugar in the diet, mass-reared insects can be easily distinguished from wild insects with a high degree of certainty. It was shown that the method could be extended using a multiple isotope approach, with 15N or a full suite of C, N, S and O isotopes. Intrinsic isotope marking of mass-reared moths proved to be an accurate means of distinguishing wild from mass-reared populations, based on isotopic differences between the wild host plant species and the diets used in mass-rearing, which where possible, had been manipulated to contain the isotopically divergent sugar type. This intrinsic labeling using stable isotopes could be useful in the assessment of the quality of mass-reared moths, because a stable isotope is a marker that does not affect the insect in any detrimental manner.

AB - In this study we identified a number of moth (Lepidoptera) species that are potential targets for the sterile insect technique (SIT), and we assessed the feasibility of using stable isotope signatures as markers to distinguish mass-reared from wild moth species. Large natural differences in the isotopic signatures of commercially available sugars render them novel markers for mass-reared insects. Sugar beet (Beta vulgaris L.; Caryophyllales: Amaranthaceae), a C3 plant, has a stable isotopic signature (a measure of the ratio of the stable isotopes 13C:12C) of around -27‰ relative to Vienna Pee Dee Belemnite (VPDB; the international C isotope standard for the stable isotopes, 13C and 12C), and sugarcane (Saccharum spp.; Poales: Poaceae), a C4 plant, has an isotopic signature of around -11‰. Thus by means of such a distinct isotope ratio in the sugar in the diet, mass-reared insects can be easily distinguished from wild insects with a high degree of certainty. It was shown that the method could be extended using a multiple isotope approach, with 15N or a full suite of C, N, S and O isotopes. Intrinsic isotope marking of mass-reared moths proved to be an accurate means of distinguishing wild from mass-reared populations, based on isotopic differences between the wild host plant species and the diets used in mass-rearing, which where possible, had been manipulated to contain the isotopically divergent sugar type. This intrinsic labeling using stable isotopes could be useful in the assessment of the quality of mass-reared moths, because a stable isotope is a marker that does not affect the insect in any detrimental manner.

KW - Elemental analysis-isotope ratio mass-spectrometry (EA-IRMS)

KW - Isotopic signature

KW - Larval diet

KW - Markers

KW - Moths

KW - Release/recapture

KW - Sterile insects

KW - Sugar

UR - http://www.scopus.com/inward/record.url?scp=84983350884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84983350884&partnerID=8YFLogxK

U2 - 10.1653/024.099.sp120

DO - 10.1653/024.099.sp120

M3 - Article

AN - SCOPUS:84983350884

VL - 99

SP - 166

EP - 176

JO - Florida Entomologist

JF - Florida Entomologist

SN - 0015-4040

ER -