Stability of lactate dehydrogenase, aspartate aminotransferase, alkaline phosphatase and tartrate resistant acid phosphatase in human saliva and gingival crevicular fluid in the presence of protease inhibitor

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Abstract

The stability of aspartate aminotransferase (AST), lactate dehydrogenase (LDH), tartrate resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) activities from saliva and gingival crevicular fluid (GCF) with and without the addition of protease inhibitor (PI) at room temperature (RT; 25°C), 4°C and -20°C were investigated. AST, LDH, TRAP and ALP activities in saliva and GCF (n=9) with and without the addition of PI were assayed at 0 (control), 12, 24, 48, 72 h, one and two weeks. A paired t-test showed there were a significant differences (p<0.05) between LDH and TRAP activities in saliva, in the presence and without PI at all temperatures. ALP activity exhibited a significant difference in activity (p<0.05) in the presence and without PI at RT while no significant differences were observed at 4°C and -20°C. A significant difference (p<0.05) was observed in AST, LDH and TRAP activities (GCF) at RT and 4°C in the presence and without PI. We conclude that PI is essential for maintaining stable enzyme activities in saliva and GCF.

Original languageEnglish
Pages (from-to)1131-1140
Number of pages10
JournalArchives of Biological Sciences
Volume65
Issue number3
DOIs
Publication statusPublished - 2013

Fingerprint

Gingival Crevicular Fluid
Aspartate Aminotransferases
Acid Phosphatase
saliva
proteinase inhibitors
Protease Inhibitors
lactate dehydrogenase
L-Lactate Dehydrogenase
Saliva
acid phosphatase
aspartate transaminase
Alkaline Phosphatase
alkaline phosphatase
Fluids
Temperature
Enzyme activity
Tartrate-Resistant Acid Phosphatase
fluids
tartaric acid
ambient temperature

Keywords

  • Gingival crevicular fluid
  • Protease inhibitor
  • Saliva

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

@article{7e38e8f13425481599d3e31269d86df0,
title = "Stability of lactate dehydrogenase, aspartate aminotransferase, alkaline phosphatase and tartrate resistant acid phosphatase in human saliva and gingival crevicular fluid in the presence of protease inhibitor",
abstract = "The stability of aspartate aminotransferase (AST), lactate dehydrogenase (LDH), tartrate resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) activities from saliva and gingival crevicular fluid (GCF) with and without the addition of protease inhibitor (PI) at room temperature (RT; 25°C), 4°C and -20°C were investigated. AST, LDH, TRAP and ALP activities in saliva and GCF (n=9) with and without the addition of PI were assayed at 0 (control), 12, 24, 48, 72 h, one and two weeks. A paired t-test showed there were a significant differences (p<0.05) between LDH and TRAP activities in saliva, in the presence and without PI at all temperatures. ALP activity exhibited a significant difference in activity (p<0.05) in the presence and without PI at RT while no significant differences were observed at 4°C and -20°C. A significant difference (p<0.05) was observed in AST, LDH and TRAP activities (GCF) at RT and 4°C in the presence and without PI. We conclude that PI is essential for maintaining stable enzyme activities in saliva and GCF.",
keywords = "Gingival crevicular fluid, Protease inhibitor, Saliva",
author = "Kasim, {Nurfathiha Abu} and {Zainal Ariffin}, {Shahrul Hisham} and Shahidan, {Muhammad Ashraf} and Abidin, {Intan Zarina Zainol} and Sahidan Senafi and Jemain, {Abdul Aziz} and {Megat Abdul Wahab}, Rohaya",
year = "2013",
doi = "10.2298/ABS1303131K",
language = "English",
volume = "65",
pages = "1131--1140",
journal = "Archives of Biological Sciences",
issn = "0354-4664",
publisher = "Institut za Bioloska Istrazivanja",
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T1 - Stability of lactate dehydrogenase, aspartate aminotransferase, alkaline phosphatase and tartrate resistant acid phosphatase in human saliva and gingival crevicular fluid in the presence of protease inhibitor

AU - Kasim, Nurfathiha Abu

AU - Zainal Ariffin, Shahrul Hisham

AU - Shahidan, Muhammad Ashraf

AU - Abidin, Intan Zarina Zainol

AU - Senafi, Sahidan

AU - Jemain, Abdul Aziz

AU - Megat Abdul Wahab, Rohaya

PY - 2013

Y1 - 2013

N2 - The stability of aspartate aminotransferase (AST), lactate dehydrogenase (LDH), tartrate resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) activities from saliva and gingival crevicular fluid (GCF) with and without the addition of protease inhibitor (PI) at room temperature (RT; 25°C), 4°C and -20°C were investigated. AST, LDH, TRAP and ALP activities in saliva and GCF (n=9) with and without the addition of PI were assayed at 0 (control), 12, 24, 48, 72 h, one and two weeks. A paired t-test showed there were a significant differences (p<0.05) between LDH and TRAP activities in saliva, in the presence and without PI at all temperatures. ALP activity exhibited a significant difference in activity (p<0.05) in the presence and without PI at RT while no significant differences were observed at 4°C and -20°C. A significant difference (p<0.05) was observed in AST, LDH and TRAP activities (GCF) at RT and 4°C in the presence and without PI. We conclude that PI is essential for maintaining stable enzyme activities in saliva and GCF.

AB - The stability of aspartate aminotransferase (AST), lactate dehydrogenase (LDH), tartrate resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) activities from saliva and gingival crevicular fluid (GCF) with and without the addition of protease inhibitor (PI) at room temperature (RT; 25°C), 4°C and -20°C were investigated. AST, LDH, TRAP and ALP activities in saliva and GCF (n=9) with and without the addition of PI were assayed at 0 (control), 12, 24, 48, 72 h, one and two weeks. A paired t-test showed there were a significant differences (p<0.05) between LDH and TRAP activities in saliva, in the presence and without PI at all temperatures. ALP activity exhibited a significant difference in activity (p<0.05) in the presence and without PI at RT while no significant differences were observed at 4°C and -20°C. A significant difference (p<0.05) was observed in AST, LDH and TRAP activities (GCF) at RT and 4°C in the presence and without PI. We conclude that PI is essential for maintaining stable enzyme activities in saliva and GCF.

KW - Gingival crevicular fluid

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