Stability Enhancement of Silver Nanoparticles Through Surface Encapsulation via a Facile Green Synthesis Approach and Toxicity Reduction

Mahmuda Akter, A. K.M.Atique Ullah, Md Shiblur Rahaman, Md Mostafizur Rahman, Md Tajuddin Sikder, Toshiyuki Hosokawa, Takeshi Saito, Masaaki Kurasaki

Research output: Contribution to journalArticle

Abstract

Persuaded by the necessity of reduction of toxicity, silver nanoparticles (Ag-NPs) were synthesized from the reaction between AgNO3 solution and Mizuna (Brassica rapa var. japonica) leaf extract. This study aimed to investigate the role of synthesis temperature on particle properties. Thus, four synthesis-temperatures, 25 °C (room temperature), 60 °C, 80 °C, and 100 °C were applied. Ag-NPs were characterized using ultraviolet–visible (UV–Vis) spectrophotometer, energy dispersive X-ray spectrometer, X-ray diffractometer, transmission electron microscope, atomic absorption spectrometry and dynamic light scattering techniques. The high-temperature (80 and 100 °C) synthesis showed higher stability, demostrated lowest release of silver ion (0.02 µg/mL, 0.002 µg/mL) compared to low-temperature synthesis. In addition, high-temperature synthesis showed higher C-content (> 50%) with lower Ag-content (< 30%) than low-temperature (25 and 60 °C) synthesis. Moreover, a complete surface encapsulation is found on the particles synthesised at 100 °C by TEM analysis. The synthesized Ag-NPs were exposed to Caco-2 cell lines for cytotoxicity assessment. The high-temperature synthesis Ag-NPs exerted significantly lower cytotoxicity in terms of Caco-2 cell viability and lactate dehydrogenase (LDH) activity assay. Upon exposure to 1, 5 and 10 µg/mL Ag-NPs for 48 h the cell viability (96.33%, 90.66% and 89.66%) was not changed for high-temperature synthesis particles as well as the LDH activity was unchanged. However, low-temperature synthesis showed significant cytotoxicity (81%, 66% and 44.33%) under similar experimental conditions. Thus, characterization and cytotoxicity techniques clearly demonstrate the successful synthesis of Ag-NPs with the encapsulation of bio-molecules that enhances the particle stability and reduce cytotoxicity.

Original languageEnglish
JournalJournal of Inorganic and Organometallic Polymers and Materials
DOIs
Publication statusAccepted/In press - 1 Jan 2019

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Encapsulation
Silver
Toxicity
Nanoparticles
Cytotoxicity
Temperature
L-Lactate Dehydrogenase
X ray spectrometers
Atomic absorption spectrometry
Spectrophotometers
Diffractometers
Dynamic light scattering
Assays
Electron microscopes
Thermodynamic properties
Cells
Ions
Transmission electron microscopy
X rays
Molecules

Keywords

  • Cytotoxicity
  • Encapsulation
  • Green synthesis
  • Silver nanoparticles
  • Stability

ASJC Scopus subject areas

  • Polymers and Plastics
  • Materials Chemistry

Cite this

Stability Enhancement of Silver Nanoparticles Through Surface Encapsulation via a Facile Green Synthesis Approach and Toxicity Reduction. / Akter, Mahmuda; Ullah, A. K.M.Atique; Rahaman, Md Shiblur; Rahman, Md Mostafizur; Sikder, Md Tajuddin; Hosokawa, Toshiyuki; Saito, Takeshi; Kurasaki, Masaaki.

In: Journal of Inorganic and Organometallic Polymers and Materials, 01.01.2019.

Research output: Contribution to journalArticle

Akter, Mahmuda ; Ullah, A. K.M.Atique ; Rahaman, Md Shiblur ; Rahman, Md Mostafizur ; Sikder, Md Tajuddin ; Hosokawa, Toshiyuki ; Saito, Takeshi ; Kurasaki, Masaaki. / Stability Enhancement of Silver Nanoparticles Through Surface Encapsulation via a Facile Green Synthesis Approach and Toxicity Reduction. In: Journal of Inorganic and Organometallic Polymers and Materials. 2019.
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AU - Rahaman, Md Shiblur

AU - Rahman, Md Mostafizur

AU - Sikder, Md Tajuddin

AU - Hosokawa, Toshiyuki

AU - Saito, Takeshi

AU - Kurasaki, Masaaki

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