Analisis spektroskopik ramnolipid yang dihasilkan oleh P. aeruginosa UKMP14T

Translated title of the contribution: Spectroscopic analysis of rhamnolipid produced by produced by Pseudomonas aeruginosa UKMP14T

Noramiza Sabturani, Jalifah Latif, Shahidan Radiman, Ainon Hamzah

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Biosurfactant produced by Pseudomonas aeruginosa UKMP14T was optimized by growing the isolate in mineral salt medium (MSM) supplemented with 1% (v/v) glycerol and 1.3 g/L ammonium sulphate with C/N ratio of 14:1. The culture medium was incubated at 37°C, with an agitation speed of 150 rpm for 7 days. P. aeruginosa UKMP14T produced biosurfactant at 0.8 g/L after 7 days incubation. Anthrone assay proved biosurfactant was glycolipid. The biosurfactant was characterized by scanning electron microscope-energy dispersive X-ray spectroscopy (SEM-EDX) in addition to Fourier transform infra-red (FT-IR), nuclear magnetic resonance (NMR) and electrospray ionization-mass spectrometry (ESI-MS). The SEM-EDX analysis indicated the presence of carbon and oxygen elements by 78% and 22% (atomic %), respectively. FT-IR absorption spectra indicated the functional groups of rhamnolipid were located at 3308.46, 2922.91, 2857.09 and 1730.96 cm-1. ESI-MS/MS analyses identified P. aeruginosa UKMP14T produced rhamnolipid with two fatty acids-nine congeners, L-rhamnosyl-L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (Rha-Rha-C10-C10) (m/z 649) which formed the main compound. The critical micelle concentration (CMC) of this rhamnolipid was established at 30 mg/L (32 dynes/cm). The characteristics of biosurfactant produced by P. aeruginosa UKMP14T indicated it has a high potential in industrial dan bioremediation application.

Original languageMalay
Pages (from-to)31-43
Number of pages13
JournalMalaysian Journal of Analytical Sciences
Volume20
Issue number1
Publication statusPublished - 2016

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Spectroscopic analysis
Electrospray ionization
Mass spectrometry
Fourier transforms
Electron microscopes
Scanning
Bioremediation
Critical micelle concentration
Glycolipids
Infrared absorption
Ammonium Sulfate
Glycerol
Functional groups
Minerals
Culture Media
Absorption spectra
Assays
Fatty Acids
Carbon
Salts

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Analisis spektroskopik ramnolipid yang dihasilkan oleh P. aeruginosa UKMP14T. / Sabturani, Noramiza; Latif, Jalifah; Radiman, Shahidan; Hamzah, Ainon.

In: Malaysian Journal of Analytical Sciences, Vol. 20, No. 1, 2016, p. 31-43.

Research output: Contribution to journalArticle

Sabturani, Noramiza ; Latif, Jalifah ; Radiman, Shahidan ; Hamzah, Ainon. / Analisis spektroskopik ramnolipid yang dihasilkan oleh P. aeruginosa UKMP14T. In: Malaysian Journal of Analytical Sciences. 2016 ; Vol. 20, No. 1. pp. 31-43.
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