Species identification of intestinal microsporidia using immunofluorescence antibody assays

M. Abdulsalam Al-Mekhlafi, M. S. Fatmah, Anisah Nordin, `Azlin Muhammad @ Mohd Yasin, Hesham M. Al-Mekhlafi, Norhayati Moktar

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The species identification of Enterocytozoon bieneusi and Encephalitozoon intestinalis is only possible using transmission electron microscopy (TEM), molecular techniques and immunofluorescence antibody assays (IFA). In this study, 50 positive and 50 negative fecal specimens for microsporidial spores using the Weber modified trichrome (WMT) staining technique were examined using IFAMAbs. Of the 100 specimens examined, the microsporidial spores identified by IFA-MAbs were Enterocytozoon bieneusi 42 (75%) Encephalitozoon intestinalis 7 (12.5%) and mixed infections 7 (12.5%). The sensitivity and specificity of IFA-MAbs in detecting microsporidial spores were 98% and 86%, respectively. The agreement between the WMT staining technique and IFA-MAbs was statistically significant by Kappa statistics (K=0.840; p<0.001). E. bieneusi was the commonest Microsporidia species isolated from the studied population; the presence of microsporidial spores detected by IFA-MAbs should be confirmed by other methods.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalSoutheast Asian Journal of Tropical Medicine and Public Health
Volume42
Issue number1
Publication statusPublished - Jan 2011

Fingerprint

Microsporidia
Enterocytozoon
Fluorescent Antibody Technique
Spores
Encephalitozoon
Antibodies
Staining and Labeling
Transmission Electron Microscopy
Coinfection
Sensitivity and Specificity
Population

Keywords

  • Identification
  • IFA-MAbs
  • Microsporidia

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health

Cite this

Species identification of intestinal microsporidia using immunofluorescence antibody assays. / Al-Mekhlafi, M. Abdulsalam; Fatmah, M. S.; Nordin, Anisah; Muhammad @ Mohd Yasin, `Azlin; Al-Mekhlafi, Hesham M.; Moktar, Norhayati.

In: Southeast Asian Journal of Tropical Medicine and Public Health, Vol. 42, No. 1, 01.2011, p. 19-24.

Research output: Contribution to journalArticle

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