Speciation and antimicrobial resistance of Enterococci isolated from recreational beaches in Malaysia

Ayokunle Christopher Dada, Asmat Ahmad, Gires Usup, Yook Heng Lee

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We report the first study on the occurrence of antibiotic-resistant enterococci in coastal bathing waters in Malaysia. One hundred and sixty-five enterococci isolates recovered from two popular recreational beaches in Malaysia were speciated and screened for antibiotic resistance to a total of eight antibiotics. Prevalence of Enterococcus faecalis and Enterococcus faecium was highest in both beaches. E. faecalis/E. faecium ratio was 0.384:1 and 0.375:1, respectively, for isolates from Port Dickson (PD) and Bagan Lalang (BL). Analysis of Fisher's exact test showed that association of prevalence of E. faecalis and E. faecium with considered locations was not statistically significant (p < 0.05). Chi-square test revealed significant differences (χ 2 = 82.630, df = 20, p < 0.001) in the frequency of occurrence of enterococci isolates from the considered sites. Resistance was highest to nalidixic acid (94.84 %) and least for chloramphenicol (8.38 %). One-way ANOVA using Tukey-Kramer multiple comparison test showed that resistance to ampicillin was higher in PD beach isolates than BL isolates and the difference was extremely statistically significant (p < 0.0001). Frequency of occurrence of multiple antibiotic resistance (MAR) isolates were higher for PD beach water (64.29 %) as compared to BL beach water (13.51 %), while MAR indices ranged between 0.198 and 0.48. The results suggest that samples from Port Dickson may contain MAR bacteria and that this could be due to high-risk faecal contamination from sewage discharge pipes that drain into the sea water.

Original languageEnglish
Pages (from-to)1583-1599
Number of pages17
JournalEnvironmental Monitoring and Assessment
Volume185
Issue number2
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Antibiotics
Beaches
antibiotic resistance
beach
antibiotics
Water
bathing water
drain
Sewage
Analysis of variance (ANOVA)
sewage
pipe
Bacteria
seawater
Contamination
water
Pipe
bacterium
acid
Acids

Keywords

  • Antimicrobial resistance
  • Beach sand
  • Enterococcus
  • Malaysia
  • Recreational beach

ASJC Scopus subject areas

  • Environmental Science(all)
  • Management, Monitoring, Policy and Law
  • Pollution

Cite this

Speciation and antimicrobial resistance of Enterococci isolated from recreational beaches in Malaysia. / Dada, Ayokunle Christopher; Ahmad, Asmat; Usup, Gires; Lee, Yook Heng.

In: Environmental Monitoring and Assessment, Vol. 185, No. 2, 02.2013, p. 1583-1599.

Research output: Contribution to journalArticle

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