Somatic embryogenesis of mangosteen

Emelda Rosseleena Rohani, Ismanizan Ismail, Normah Mohd. Noor

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Young red leaf segments from 1 to 3 year-old seedlings of mangosteen (Garcinia mangostana L.) were incubated on different media, consisting of either B5 or Murashige and Skoog (MS) basal media and supplemented with several plant growth regulator at different levels, including 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin or 2,4-D and 6-benzyladenine (BA) or thidiazuron (TDZ) and BA. The highest percentage of globular structures (36.0 %) was obtained using MS medium with a combination of TDZ and BA, each at a concentration of 0.7 mg l -1. Histological observation of sections of globular structures showed accumulation of dense meristematic cells, a common characteristic of globular formation during somatic embryogenesis. Molecular analysis detected the gene somatic embryogenesis receptor-like kinase 1 (SERK1). This gene sequence showed a high similarity (73.0-87.0 %) to previously reported plant SERK1.

Original languageEnglish
Pages (from-to)251-259
Number of pages9
JournalPlant Cell, Tissue and Organ Culture
Volume110
Issue number2
DOIs
Publication statusPublished - Aug 2012

Fingerprint

mangosteens
somatic embryogenesis
benzyladenine
2,4-D
phosphotransferases (kinases)
Garcinia mangostana
receptors
kinetin
plant growth substances
nucleotide sequences
seedlings
leaves
genes
cells
thidiazuron

Keywords

  • Globular structures
  • Histological observation
  • Mangosteen
  • Molecular analysis
  • Somatic embryogenesis receptor-like kinase 1

ASJC Scopus subject areas

  • Horticulture

Cite this

Somatic embryogenesis of mangosteen. / Rohani, Emelda Rosseleena; Ismail, Ismanizan; Mohd. Noor, Normah.

In: Plant Cell, Tissue and Organ Culture, Vol. 110, No. 2, 08.2012, p. 251-259.

Research output: Contribution to journalArticle

Rohani, Emelda Rosseleena ; Ismail, Ismanizan ; Mohd. Noor, Normah. / Somatic embryogenesis of mangosteen. In: Plant Cell, Tissue and Organ Culture. 2012 ; Vol. 110, No. 2. pp. 251-259.
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