Somatic Embryogenesis from Scutellar Embryo of Oryza sativa L. var. MR219

Syaiful Bahri Panjaitan, Siti Nor Akmar Abdullah, Maheran Abdul Aziz, Sariah Meon, Othman Omar

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Somatic embryogenesis is an efficient plant regeneration system and it is a potentially useful tool for genetic transformation. An experiment was carried out on somatic embryogenesis from scutellar embryo of rice var. MR219. High intensity of callus formation (100%) was initiated through culturing the scutellar embryo on modified MS medium, with the macro nutrients reduced to half-strength and supplemented with different 2,4-D concentrations (1,2,4 and 6 mgL-1). Meanwhile, the highest percentage of embryogénie callus formation (80%) was obtained on the modified MS medium containing 4 mgL-12, 4-D. The calli produced were yellowish and friable with nodular structures on the surface. Rounded cells with highly dense cytoplasm were also observed under an inverted microscope and their viability was confirmed based on the apple green fluorescence staining in the fluorescein diacetate (FDA) solution. High mean number of somatic embryos was also produced in this treatment, at 85 somatic embryos per expiant. Upon transferring the somatic embryos onto the modified MS medium with 2 mgL-1 BAP and 0.05 mgL-1 NAA for germination, 82.5% of the somatic embryos were germinated into the seedlings.

Original languageEnglish
Pages (from-to)185-194
Number of pages10
JournalPertanika Journal of Tropical Agricultural Science
Volume32
Issue number2
Publication statusPublished - Aug 2009
Externally publishedYes

Fingerprint

somatic embryos
somatic embryogenesis
embryo (plant)
Oryza sativa
callus formation
genetic transformation
fluorescein
microscopes
2,4-D
callus
cytoplasm
apples
viability
fluorescence
germination
rice
seedlings
nutrients
cells

Keywords

  • α-naphthalenaceric acid
  • 2,4-dichlorophenoxyacetic acid
  • 6-benzylaminopurine
  • Rice (oryza sativa l.) var. Mr219

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Panjaitan, S. B., Abdullah, S. N. A., Aziz, M. A., Meon, S., & Omar, O. (2009). Somatic Embryogenesis from Scutellar Embryo of Oryza sativa L. var. MR219. Pertanika Journal of Tropical Agricultural Science, 32(2), 185-194.

Somatic Embryogenesis from Scutellar Embryo of Oryza sativa L. var. MR219. / Panjaitan, Syaiful Bahri; Abdullah, Siti Nor Akmar; Aziz, Maheran Abdul; Meon, Sariah; Omar, Othman.

In: Pertanika Journal of Tropical Agricultural Science, Vol. 32, No. 2, 08.2009, p. 185-194.

Research output: Contribution to journalArticle

Panjaitan, SB, Abdullah, SNA, Aziz, MA, Meon, S & Omar, O 2009, 'Somatic Embryogenesis from Scutellar Embryo of Oryza sativa L. var. MR219', Pertanika Journal of Tropical Agricultural Science, vol. 32, no. 2, pp. 185-194.
Panjaitan, Syaiful Bahri ; Abdullah, Siti Nor Akmar ; Aziz, Maheran Abdul ; Meon, Sariah ; Omar, Othman. / Somatic Embryogenesis from Scutellar Embryo of Oryza sativa L. var. MR219. In: Pertanika Journal of Tropical Agricultural Science. 2009 ; Vol. 32, No. 2. pp. 185-194.
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