Solution Structures, Dynamics, and Ice Growth Inhibitory Activity of Peptide Fragments Derived from an Antarctic Yeast Protein

Syed Hussinien H Shah, Rajiv K. Kar, Azren A. Asmawi, Mohd Basyaruddin A Rahman, Abdul Munir Abd. Murad, Nor M. Mahadi, Mahiran Basri, Raja Noor Zaliha A Rahman, Abu B. Salleh, Subhrangsu Chatterjee, Bimo A. Tejo, Anirban Bhunia

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Exotic functions of antifreeze proteins (AFP) and antifreeze glycopeptides (AFGP) have recently been attracted with much interest to develop them as commercial products. AFPs and AFGPs inhibit ice crystal growth by lowering the water freezing point without changing the water melting point. Our group isolated the Antarctic yeast Glaciozyma antarctica that expresses antifreeze protein to assist it in its survival mechanism at sub-zero temperatures. The protein is unique and novel, indicated by its low sequence homology compared to those of other AFPs. We explore the structure-function relationship of G. antarctica AFP using various approaches ranging from protein structure prediction, peptide design and antifreeze activity assays, nuclear magnetic resonance (NMR) studies and molecular dynamics simulation. The predicted secondary structure of G. antarctica AFP shows several α-helices, assumed to be responsible for its antifreeze activity. We designed several peptide fragments derived from the amino acid sequences of α-helical regions of the parent AFP and they also showed substantial antifreeze activities, below that of the original AFP. The relationship between peptide structure and activity was explored by NMR spectroscopy and molecular dynamics simulation. NMR results show that the antifreeze activity of the peptides correlates with their helicity and geometrical straightforwardness. Furthermore, molecular dynamics simulation also suggests that the activity of the designed peptides can be explained in terms of the structural rigidity/flexibility, i.e., the most active peptide demonstrates higher structural stability, lower flexibility than that of the other peptides with lower activities, and of lower rigidity. This report represents the first detailed report of downsizing a yeast AFP into its peptide fragments with measurable antifreeze activities.

Original languageEnglish
Article numbere49788
JournalPLoS One
Volume7
Issue number11
DOIs
Publication statusPublished - 28 Nov 2012

Fingerprint

Antifreeze Proteins
Peptide Fragments
Fungal Proteins
Ice
ice
peptides
yeasts
Growth
molecular dynamics
proteins
Antarctica
nuclear magnetic resonance spectroscopy
Peptides
Molecular Dynamics Simulation
Molecular dynamics
Magnetic Resonance Spectroscopy
Rigidity
Yeast
Freezing
Computer simulation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Solution Structures, Dynamics, and Ice Growth Inhibitory Activity of Peptide Fragments Derived from an Antarctic Yeast Protein. / Shah, Syed Hussinien H; Kar, Rajiv K.; Asmawi, Azren A.; Rahman, Mohd Basyaruddin A; Abd. Murad, Abdul Munir; Mahadi, Nor M.; Basri, Mahiran; Rahman, Raja Noor Zaliha A; Salleh, Abu B.; Chatterjee, Subhrangsu; Tejo, Bimo A.; Bhunia, Anirban.

In: PLoS One, Vol. 7, No. 11, e49788, 28.11.2012.

Research output: Contribution to journalArticle

Shah, SHH, Kar, RK, Asmawi, AA, Rahman, MBA, Abd. Murad, AM, Mahadi, NM, Basri, M, Rahman, RNZA, Salleh, AB, Chatterjee, S, Tejo, BA & Bhunia, A 2012, 'Solution Structures, Dynamics, and Ice Growth Inhibitory Activity of Peptide Fragments Derived from an Antarctic Yeast Protein', PLoS One, vol. 7, no. 11, e49788. https://doi.org/10.1371/journal.pone.0049788
Shah, Syed Hussinien H ; Kar, Rajiv K. ; Asmawi, Azren A. ; Rahman, Mohd Basyaruddin A ; Abd. Murad, Abdul Munir ; Mahadi, Nor M. ; Basri, Mahiran ; Rahman, Raja Noor Zaliha A ; Salleh, Abu B. ; Chatterjee, Subhrangsu ; Tejo, Bimo A. ; Bhunia, Anirban. / Solution Structures, Dynamics, and Ice Growth Inhibitory Activity of Peptide Fragments Derived from an Antarctic Yeast Protein. In: PLoS One. 2012 ; Vol. 7, No. 11.
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