Solid phase microextraction based micro-device for extraction of PCR amplifiable DNA

G. Samla, Gan Kok Beng, S. M. Then

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Deoxyribonucleic acid (DNA) extraction or sample preparation from biological sample (whole blood) for downstream process on microfluidic platform has been widely studied due to its crucial role in clinical diagnostic and genetic analysis. Sample preparation can be complicated since blood comprises complex matrices and repeated blood pricking from patients can be affected their health. Thus, introduction of solid-phase microextraction (SPME) method would be suitable to isolate, fractionate and concentrate the analyte from complex sample matrices and implemented on microfluidic device with small amount of sample. SPME is an effective sample preparation method by chemical lysis followed by purification mostly by using silica-based platform developed based on direct absorption into silica resins and desorption of analyte from silica resins.

Original languageEnglish
Pages (from-to)63-80
Number of pages18
JournalInternational Journal of Nanoelectronics and Materials
Volume10
Issue number1
Publication statusPublished - 2017

Fingerprint

Silicon Dioxide
DNA
Blood
Silica
Microfluidics
Resins
Purification
Desorption
Health

Keywords

  • DNA extraction
  • Micro-device
  • SPME
  • Whole blood

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering

Cite this

Solid phase microextraction based micro-device for extraction of PCR amplifiable DNA. / Samla, G.; Kok Beng, Gan; Then, S. M.

In: International Journal of Nanoelectronics and Materials, Vol. 10, No. 1, 2017, p. 63-80.

Research output: Contribution to journalArticle

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