Sodium carboxymethyl cellulose hydrogels containing reduced graphene oxide (rGO) as a functional antibiofilm wound dressing

Research output: Contribution to journalArticle

Abstract

Biofilms comprise bacteria attached to wound surfaces and are major contributors to non-healing wounds. It was found that the increased resistance of biofilms to antibiotics allows wound infections to persist chronically in spite of antibiotic therapy. In this study, the reduced form of graphene oxide (rGO) was explored as plausible antibiofilm agents. The rGO was synthesized via reducing the functional groups of GO. Then, rGO were characterized using zetasizer, X-ray photoelectron spectroscopy, UV–Vis spectroscopy and FESEM. The rGO were then formulated into sodium carboxymethyl cellulose (NaCMC) hydrogels to form rGO hydrogel and tested for antibiofilm activities in vitro using XTT test, and in vivo biofilm formation assay using nematodes C. elegans. Reduced GO hydrogel was successfully formed by reducing the functional groups of GO, and a reduction of up to 95% of functional groups was confirmed with X-ray photoelectron spectroscopy analysis. XTT tests confirmed that rGO hydrogels reduced biofilm formation by S. aureus (81–84%) and P. aeruginosa (50–62%). Fluorescence intensity also confirmed that rGO hydrogel can inhibit biofilm bacteria in C. elegans experiments. This study implied that rGO hydrogel is an effective antibiofilm agent for infected wounds.

Original languageEnglish
JournalJournal of Biomaterials Science, Polymer Edition
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Carboxymethylcellulose Sodium
Hydrogels
Graphite
Bandages
Oxides
Graphene
Cellulose
Sodium
Biofilms
Hydrogel
Wounds and Injuries
Functional groups
Photoelectron Spectroscopy
Antibiotics
Bacteria
X ray photoelectron spectroscopy
Anti-Bacterial Agents
Wound Infection
Assays
Spectrum Analysis

Keywords

  • antibiofilm
  • Reduced graphene oxide
  • sodium carboxymethyl cellulose
  • wound dressing

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

@article{8a86877aba7347bf869e36ea6ae55b9c,
title = "Sodium carboxymethyl cellulose hydrogels containing reduced graphene oxide (rGO) as a functional antibiofilm wound dressing",
abstract = "Biofilms comprise bacteria attached to wound surfaces and are major contributors to non-healing wounds. It was found that the increased resistance of biofilms to antibiotics allows wound infections to persist chronically in spite of antibiotic therapy. In this study, the reduced form of graphene oxide (rGO) was explored as plausible antibiofilm agents. The rGO was synthesized via reducing the functional groups of GO. Then, rGO were characterized using zetasizer, X-ray photoelectron spectroscopy, UV–Vis spectroscopy and FESEM. The rGO were then formulated into sodium carboxymethyl cellulose (NaCMC) hydrogels to form rGO hydrogel and tested for antibiofilm activities in vitro using XTT test, and in vivo biofilm formation assay using nematodes C. elegans. Reduced GO hydrogel was successfully formed by reducing the functional groups of GO, and a reduction of up to 95{\%} of functional groups was confirmed with X-ray photoelectron spectroscopy analysis. XTT tests confirmed that rGO hydrogels reduced biofilm formation by S. aureus (81–84{\%}) and P. aeruginosa (50–62{\%}). Fluorescence intensity also confirmed that rGO hydrogel can inhibit biofilm bacteria in C. elegans experiments. This study implied that rGO hydrogel is an effective antibiofilm agent for infected wounds.",
keywords = "antibiofilm, Reduced graphene oxide, sodium carboxymethyl cellulose, wound dressing",
author = "Ali, {Nor Hazwan} and {Mohd Amin}, {Mohd Cairul Iqbal} and Ng, {Shiow Fern}",
year = "2019",
month = "1",
day = "1",
doi = "10.1080/09205063.2019.1595892",
language = "English",
journal = "Journal of Adhesion Science and Technology",
issn = "0169-4243",
publisher = "Taylor and Francis Ltd.",

}

TY - JOUR

T1 - Sodium carboxymethyl cellulose hydrogels containing reduced graphene oxide (rGO) as a functional antibiofilm wound dressing

AU - Ali, Nor Hazwan

AU - Mohd Amin, Mohd Cairul Iqbal

AU - Ng, Shiow Fern

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Biofilms comprise bacteria attached to wound surfaces and are major contributors to non-healing wounds. It was found that the increased resistance of biofilms to antibiotics allows wound infections to persist chronically in spite of antibiotic therapy. In this study, the reduced form of graphene oxide (rGO) was explored as plausible antibiofilm agents. The rGO was synthesized via reducing the functional groups of GO. Then, rGO were characterized using zetasizer, X-ray photoelectron spectroscopy, UV–Vis spectroscopy and FESEM. The rGO were then formulated into sodium carboxymethyl cellulose (NaCMC) hydrogels to form rGO hydrogel and tested for antibiofilm activities in vitro using XTT test, and in vivo biofilm formation assay using nematodes C. elegans. Reduced GO hydrogel was successfully formed by reducing the functional groups of GO, and a reduction of up to 95% of functional groups was confirmed with X-ray photoelectron spectroscopy analysis. XTT tests confirmed that rGO hydrogels reduced biofilm formation by S. aureus (81–84%) and P. aeruginosa (50–62%). Fluorescence intensity also confirmed that rGO hydrogel can inhibit biofilm bacteria in C. elegans experiments. This study implied that rGO hydrogel is an effective antibiofilm agent for infected wounds.

AB - Biofilms comprise bacteria attached to wound surfaces and are major contributors to non-healing wounds. It was found that the increased resistance of biofilms to antibiotics allows wound infections to persist chronically in spite of antibiotic therapy. In this study, the reduced form of graphene oxide (rGO) was explored as plausible antibiofilm agents. The rGO was synthesized via reducing the functional groups of GO. Then, rGO were characterized using zetasizer, X-ray photoelectron spectroscopy, UV–Vis spectroscopy and FESEM. The rGO were then formulated into sodium carboxymethyl cellulose (NaCMC) hydrogels to form rGO hydrogel and tested for antibiofilm activities in vitro using XTT test, and in vivo biofilm formation assay using nematodes C. elegans. Reduced GO hydrogel was successfully formed by reducing the functional groups of GO, and a reduction of up to 95% of functional groups was confirmed with X-ray photoelectron spectroscopy analysis. XTT tests confirmed that rGO hydrogels reduced biofilm formation by S. aureus (81–84%) and P. aeruginosa (50–62%). Fluorescence intensity also confirmed that rGO hydrogel can inhibit biofilm bacteria in C. elegans experiments. This study implied that rGO hydrogel is an effective antibiofilm agent for infected wounds.

KW - antibiofilm

KW - Reduced graphene oxide

KW - sodium carboxymethyl cellulose

KW - wound dressing

UR - http://www.scopus.com/inward/record.url?scp=85063942805&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063942805&partnerID=8YFLogxK

U2 - 10.1080/09205063.2019.1595892

DO - 10.1080/09205063.2019.1595892

M3 - Article

JO - Journal of Adhesion Science and Technology

JF - Journal of Adhesion Science and Technology

SN - 0169-4243

ER -