Sinomonas humi sp. nov., an amylolytic actinobacterium isolated from mangrove forest soil

Learn Han Lee, Adzzie Shazleen Azman, Nurullhudda Zainal, Wai Fong Yin, Nurul Syakima Ab Mutalib, Kok Gan Chan

Research output: Contribution to journalArticle

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Abstract

Strain MUSC 117Twas isolated from mangrove soil of the Tanjung Lumpur forest in Pahang, Malaysia. This bacterium was yellowish-white pigmented, Gram-staining-positive, rod–coccus shaped and non-motile. On the basis of 16S rRNA gene sequence, strain MUSC 117Texhibited highest sequence similarity to Sinomonas atrocyanea DSM 20127T(98.0%), Sinomonas albida LC13T(97.9%) and Sinomonas soli CW 59T (97.8%), and lower (<97.6%) sequence similarity to other species of the genus Sinomonas. DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 27%) between strain MUSC 117Tand closely related species. Chemotaxonomically, the peptidoglycan type was A3α, containing the amino acids lysine, serine, glycine, alanine, glutamic acid and muramic acid. The whole-cell sugars detected were rhamnose, ribose, glucose, galactose and a smaller amount of mannose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and five unidentified glycolipids. The major fatty acids (.10.0%) of the cell membrane were anteiso-C15: 0(39.4%), C18: 1ω7c (17.7%), anteiso-C17: 0(17.2%) and iso-C16: 0(11.4%). The predominant respiratory quinones detected were MK-9(H2) and MK-9. The DNA G+C content was 67.3 mol%. A comparison of BOX-PCR fingerprints indicated that strain MUSC 117Trepresented a unique DNA profile. Results based on a polyphasic approach showed that strain MUSC 117Trepresents a novel species of the genus Sinomonas, for which the name Sinomonas humi sp. nov. is proposed. The type strain of Sinomonas humi sp. nov. is MUSC 117T(5DSM 29362T=MCCC 1K00410T=NBRC 110653T).

Original languageEnglish
Pages (from-to)996-1002
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
Issue number3
DOIs
Publication statusPublished - 2015

Fingerprint

mangrove soils
Wetlands
Actinobacteria
mangrove forests
forest soils
mangrove
forest soil
Muramic Acids
Soil
Rhamnose
Quinones
Phosphatidylglycerols
Cardiolipins
Ribose
Peptidoglycan
new species
DNA
Glycolipids
Malaysia
Base Composition

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Sinomonas humi sp. nov., an amylolytic actinobacterium isolated from mangrove forest soil. / Lee, Learn Han; Azman, Adzzie Shazleen; Zainal, Nurullhudda; Yin, Wai Fong; Ab Mutalib, Nurul Syakima; Chan, Kok Gan.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, No. 3, 2015, p. 996-1002.

Research output: Contribution to journalArticle

Lee, Learn Han ; Azman, Adzzie Shazleen ; Zainal, Nurullhudda ; Yin, Wai Fong ; Ab Mutalib, Nurul Syakima ; Chan, Kok Gan. / Sinomonas humi sp. nov., an amylolytic actinobacterium isolated from mangrove forest soil. In: International Journal of Systematic and Evolutionary Microbiology. 2015 ; Vol. 65, No. 3. pp. 996-1002.
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abstract = "Strain MUSC 117Twas isolated from mangrove soil of the Tanjung Lumpur forest in Pahang, Malaysia. This bacterium was yellowish-white pigmented, Gram-staining-positive, rod–coccus shaped and non-motile. On the basis of 16S rRNA gene sequence, strain MUSC 117Texhibited highest sequence similarity to Sinomonas atrocyanea DSM 20127T(98.0{\%}), Sinomonas albida LC13T(97.9{\%}) and Sinomonas soli CW 59T (97.8{\%}), and lower (<97.6{\%}) sequence similarity to other species of the genus Sinomonas. DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 27{\%}) between strain MUSC 117Tand closely related species. Chemotaxonomically, the peptidoglycan type was A3α, containing the amino acids lysine, serine, glycine, alanine, glutamic acid and muramic acid. The whole-cell sugars detected were rhamnose, ribose, glucose, galactose and a smaller amount of mannose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and five unidentified glycolipids. The major fatty acids (.10.0{\%}) of the cell membrane were anteiso-C15: 0(39.4{\%}), C18: 1ω7c (17.7{\%}), anteiso-C17: 0(17.2{\%}) and iso-C16: 0(11.4{\%}). The predominant respiratory quinones detected were MK-9(H2) and MK-9. The DNA G+C content was 67.3 mol{\%}. A comparison of BOX-PCR fingerprints indicated that strain MUSC 117Trepresented a unique DNA profile. Results based on a polyphasic approach showed that strain MUSC 117Trepresents a novel species of the genus Sinomonas, for which the name Sinomonas humi sp. nov. is proposed. The type strain of Sinomonas humi sp. nov. is MUSC 117T(5DSM 29362T=MCCC 1K00410T=NBRC 110653T).",
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