Single spore isolation and morphological characterization of local Malaysian isolates of rice blast fungus Magnoporthe grisea

Ankitta Mishra, R Wickneswari V Ratnam, Md Atiqur Rahman Bhuiyan, Ariane Ponaya, Khisord K. Jena

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Rice blast is a destructive disease, caused by the fungal pathogen Magnaporthe grisea. It causes considerable damage to rice and leads to crop loss in rice growing regions worldwide. Although fungicides can be used to control rice blast, they generate additional cost in rice production and contamination of environment and food. Therefore, the use of resistant varieties is thought to be one of the most economically and environmentally efficient ways of crop protection from the disease. Six new local Malaysian isolates of M. grisea were isolated using single spore isolation method. Five isolates were from infected leaf samples collected from Kompleks Latihan MADA, Kedah and one was from Kelantan. These isolates were identified using morphological characteristics and microscopic studies and later confirmed by ITSequences. These isolates were induced to sporulate and used for greenhouse screening on two differential rice varieties: Mahsuri (susceptible) and Pongsu Seribu 2 (resistant). Among the 6 isolates, isolate number 3 was found to be the most virulent showing high sporulation while isolate number 4 was very slow growing, and the least virulent.

Original languageEnglish
Title of host publication2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1678
ISBN (Electronic)9780735413252
DOIs
Publication statusPublished - 25 Sep 2015
Event2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015 - Selangor, Malaysia
Duration: 15 Apr 201516 Apr 2015

Other

Other2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015
CountryMalaysia
CitySelangor
Period15/4/1516/4/15

Fingerprint

spores
fungi
rice
blasts
isolation
crops
fungicides
pathogens
greenhouses
food
leaves
contamination
screening
damage
costs
causes

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Mishra, A., V Ratnam, R. W., Bhuiyan, M. A. R., Ponaya, A., & Jena, K. K. (2015). Single spore isolation and morphological characterization of local Malaysian isolates of rice blast fungus Magnoporthe grisea. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium (Vol. 1678). [020019] American Institute of Physics Inc.. https://doi.org/10.1063/1.4931204

Single spore isolation and morphological characterization of local Malaysian isolates of rice blast fungus Magnoporthe grisea. / Mishra, Ankitta; V Ratnam, R Wickneswari; Bhuiyan, Md Atiqur Rahman; Ponaya, Ariane; Jena, Khisord K.

2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015. 020019.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Mishra, A, V Ratnam, RW, Bhuiyan, MAR, Ponaya, A & Jena, KK 2015, Single spore isolation and morphological characterization of local Malaysian isolates of rice blast fungus Magnoporthe grisea. in 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. vol. 1678, 020019, American Institute of Physics Inc., 2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015, Selangor, Malaysia, 15/4/15. https://doi.org/10.1063/1.4931204
Mishra A, V Ratnam RW, Bhuiyan MAR, Ponaya A, Jena KK. Single spore isolation and morphological characterization of local Malaysian isolates of rice blast fungus Magnoporthe grisea. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678. American Institute of Physics Inc. 2015. 020019 https://doi.org/10.1063/1.4931204
Mishra, Ankitta ; V Ratnam, R Wickneswari ; Bhuiyan, Md Atiqur Rahman ; Ponaya, Ariane ; Jena, Khisord K. / Single spore isolation and morphological characterization of local Malaysian isolates of rice blast fungus Magnoporthe grisea. 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015.
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