Simultaneous analysis of 25 trace elements in micro volume of human serum by inductively coupled plasma mass spectrometry (ICP-MS)

Research output: Contribution to journalArticle

Abstract

Introduction: In recent years, trace elements have gained importance as biomarkers in many chronic diseases. Unfortunately, the requirement for sample volume increases with the extent of investigation either for diagnosis or elucidating the mechanism of the disease. Here, we describe the method development and validation for simultaneous determination of 25 trace elements (lithium [Li], beryllium [Be], magnesium [Mg], aluminium [Al], vanadium [V], chromium [Cr], manganese [Mn], iron [Fe], cobalt [Co], nickel [Ni], copper [Cu], zinc [Zn], gallium [Ga], arsenic [As], selenium [Se], rubidium [Rb], strontium [Sr], silver [Ag], cadmium [Cd], caesium [Cs], barium [Ba], mercury [Hg], thallium [Tl], lead [Pb], uranium [U]) using only 20 μL of human serum. Methods: Serum samples were digested with nitric acid and hydrochloric acid (ratio 1:1, v/v) and analysed by inductively coupled plasma–mass spectrometry (ICP-MS). Seronorm®, a human-derived serum control material was used as quality control samples. Results: The coefficient of variations for both intra- and inter-day precisions were consistently <15% for all elements. The validated method was later tested on 30 human serum samples to evaluate its applicability. Conclusion: We have successfully developed and validated a precise and accurate analytical method for determining 25 trace elements requiring very low volume of human serum.

Original languageEnglish
Article numbere00142
JournalPractical Laboratory Medicine
Volume18
DOIs
Publication statusPublished - Jan 2020

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Inductively coupled plasma mass spectrometry
Trace Elements
Mass Spectrometry
Serum
Rubidium
Beryllium
Nitric Acid
Vanadium
Cesium
Strontium
Gallium
Uranium
Hydrochloric Acid
Thallium
Arsenic
Biomarkers
Chromium
Barium
Manganese
Selenium

Keywords

  • Acid digestion
  • ICP-MS
  • Serum
  • Trace element

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

Cite this

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title = "Simultaneous analysis of 25 trace elements in micro volume of human serum by inductively coupled plasma mass spectrometry (ICP-MS)",
abstract = "Introduction: In recent years, trace elements have gained importance as biomarkers in many chronic diseases. Unfortunately, the requirement for sample volume increases with the extent of investigation either for diagnosis or elucidating the mechanism of the disease. Here, we describe the method development and validation for simultaneous determination of 25 trace elements (lithium [Li], beryllium [Be], magnesium [Mg], aluminium [Al], vanadium [V], chromium [Cr], manganese [Mn], iron [Fe], cobalt [Co], nickel [Ni], copper [Cu], zinc [Zn], gallium [Ga], arsenic [As], selenium [Se], rubidium [Rb], strontium [Sr], silver [Ag], cadmium [Cd], caesium [Cs], barium [Ba], mercury [Hg], thallium [Tl], lead [Pb], uranium [U]) using only 20 μL of human serum. Methods: Serum samples were digested with nitric acid and hydrochloric acid (ratio 1:1, v/v) and analysed by inductively coupled plasma–mass spectrometry (ICP-MS). Seronorm{\circledR}, a human-derived serum control material was used as quality control samples. Results: The coefficient of variations for both intra- and inter-day precisions were consistently <15{\%} for all elements. The validated method was later tested on 30 human serum samples to evaluate its applicability. Conclusion: We have successfully developed and validated a precise and accurate analytical method for determining 25 trace elements requiring very low volume of human serum.",
keywords = "Acid digestion, ICP-MS, Serum, Trace element",
author = "{Mohammed Nawi}, Azmawati and Chin, {Siok Fong} and Rahman Jamal",
year = "2020",
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journal = "Practical Laboratory Medicine",
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T1 - Simultaneous analysis of 25 trace elements in micro volume of human serum by inductively coupled plasma mass spectrometry (ICP-MS)

AU - Mohammed Nawi, Azmawati

AU - Chin, Siok Fong

AU - Jamal, Rahman

PY - 2020/1

Y1 - 2020/1

N2 - Introduction: In recent years, trace elements have gained importance as biomarkers in many chronic diseases. Unfortunately, the requirement for sample volume increases with the extent of investigation either for diagnosis or elucidating the mechanism of the disease. Here, we describe the method development and validation for simultaneous determination of 25 trace elements (lithium [Li], beryllium [Be], magnesium [Mg], aluminium [Al], vanadium [V], chromium [Cr], manganese [Mn], iron [Fe], cobalt [Co], nickel [Ni], copper [Cu], zinc [Zn], gallium [Ga], arsenic [As], selenium [Se], rubidium [Rb], strontium [Sr], silver [Ag], cadmium [Cd], caesium [Cs], barium [Ba], mercury [Hg], thallium [Tl], lead [Pb], uranium [U]) using only 20 μL of human serum. Methods: Serum samples were digested with nitric acid and hydrochloric acid (ratio 1:1, v/v) and analysed by inductively coupled plasma–mass spectrometry (ICP-MS). Seronorm®, a human-derived serum control material was used as quality control samples. Results: The coefficient of variations for both intra- and inter-day precisions were consistently <15% for all elements. The validated method was later tested on 30 human serum samples to evaluate its applicability. Conclusion: We have successfully developed and validated a precise and accurate analytical method for determining 25 trace elements requiring very low volume of human serum.

AB - Introduction: In recent years, trace elements have gained importance as biomarkers in many chronic diseases. Unfortunately, the requirement for sample volume increases with the extent of investigation either for diagnosis or elucidating the mechanism of the disease. Here, we describe the method development and validation for simultaneous determination of 25 trace elements (lithium [Li], beryllium [Be], magnesium [Mg], aluminium [Al], vanadium [V], chromium [Cr], manganese [Mn], iron [Fe], cobalt [Co], nickel [Ni], copper [Cu], zinc [Zn], gallium [Ga], arsenic [As], selenium [Se], rubidium [Rb], strontium [Sr], silver [Ag], cadmium [Cd], caesium [Cs], barium [Ba], mercury [Hg], thallium [Tl], lead [Pb], uranium [U]) using only 20 μL of human serum. Methods: Serum samples were digested with nitric acid and hydrochloric acid (ratio 1:1, v/v) and analysed by inductively coupled plasma–mass spectrometry (ICP-MS). Seronorm®, a human-derived serum control material was used as quality control samples. Results: The coefficient of variations for both intra- and inter-day precisions were consistently <15% for all elements. The validated method was later tested on 30 human serum samples to evaluate its applicability. Conclusion: We have successfully developed and validated a precise and accurate analytical method for determining 25 trace elements requiring very low volume of human serum.

KW - Acid digestion

KW - ICP-MS

KW - Serum

KW - Trace element

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