Abstract
Introduction: In recent years, trace elements have gained importance as biomarkers in many chronic diseases. Unfortunately, the requirement for sample volume increases with the extent of investigation either for diagnosis or elucidating the mechanism of the disease. Here, we describe the method development and validation for simultaneous determination of 25 trace elements (lithium [Li], beryllium [Be], magnesium [Mg], aluminium [Al], vanadium [V], chromium [Cr], manganese [Mn], iron [Fe], cobalt [Co], nickel [Ni], copper [Cu], zinc [Zn], gallium [Ga], arsenic [As], selenium [Se], rubidium [Rb], strontium [Sr], silver [Ag], cadmium [Cd], caesium [Cs], barium [Ba], mercury [Hg], thallium [Tl], lead [Pb], uranium [U]) using only 20 μL of human serum. Methods: Serum samples were digested with nitric acid and hydrochloric acid (ratio 1:1, v/v) and analysed by inductively coupled plasma–mass spectrometry (ICP-MS). Seronorm®, a human-derived serum control material was used as quality control samples. Results: The coefficient of variations for both intra- and inter-day precisions were consistently <15% for all elements. The validated method was later tested on 30 human serum samples to evaluate its applicability. Conclusion: We have successfully developed and validated a precise and accurate analytical method for determining 25 trace elements requiring very low volume of human serum.
| Original language | English |
|---|---|
| Article number | e00142 |
| Journal | Practical Laboratory Medicine |
| Volume | 18 |
| DOIs | |
| Publication status | Published - Jan 2020 |
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Keywords
- Acid digestion
- ICP-MS
- Serum
- Trace element
ASJC Scopus subject areas
- Radiological and Ultrasound Technology
- Clinical Biochemistry
Cite this
Simultaneous analysis of 25 trace elements in micro volume of human serum by inductively coupled plasma mass spectrometry (ICP-MS). / Mohammed Nawi, Azmawati; Chin, Siok Fong; Jamal, Rahman.
In: Practical Laboratory Medicine, Vol. 18, e00142, 01.2020.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simultaneous analysis of 25 trace elements in micro volume of human serum by inductively coupled plasma mass spectrometry (ICP-MS)
AU - Mohammed Nawi, Azmawati
AU - Chin, Siok Fong
AU - Jamal, Rahman
PY - 2020/1
Y1 - 2020/1
N2 - Introduction: In recent years, trace elements have gained importance as biomarkers in many chronic diseases. Unfortunately, the requirement for sample volume increases with the extent of investigation either for diagnosis or elucidating the mechanism of the disease. Here, we describe the method development and validation for simultaneous determination of 25 trace elements (lithium [Li], beryllium [Be], magnesium [Mg], aluminium [Al], vanadium [V], chromium [Cr], manganese [Mn], iron [Fe], cobalt [Co], nickel [Ni], copper [Cu], zinc [Zn], gallium [Ga], arsenic [As], selenium [Se], rubidium [Rb], strontium [Sr], silver [Ag], cadmium [Cd], caesium [Cs], barium [Ba], mercury [Hg], thallium [Tl], lead [Pb], uranium [U]) using only 20 μL of human serum. Methods: Serum samples were digested with nitric acid and hydrochloric acid (ratio 1:1, v/v) and analysed by inductively coupled plasma–mass spectrometry (ICP-MS). Seronorm®, a human-derived serum control material was used as quality control samples. Results: The coefficient of variations for both intra- and inter-day precisions were consistently <15% for all elements. The validated method was later tested on 30 human serum samples to evaluate its applicability. Conclusion: We have successfully developed and validated a precise and accurate analytical method for determining 25 trace elements requiring very low volume of human serum.
AB - Introduction: In recent years, trace elements have gained importance as biomarkers in many chronic diseases. Unfortunately, the requirement for sample volume increases with the extent of investigation either for diagnosis or elucidating the mechanism of the disease. Here, we describe the method development and validation for simultaneous determination of 25 trace elements (lithium [Li], beryllium [Be], magnesium [Mg], aluminium [Al], vanadium [V], chromium [Cr], manganese [Mn], iron [Fe], cobalt [Co], nickel [Ni], copper [Cu], zinc [Zn], gallium [Ga], arsenic [As], selenium [Se], rubidium [Rb], strontium [Sr], silver [Ag], cadmium [Cd], caesium [Cs], barium [Ba], mercury [Hg], thallium [Tl], lead [Pb], uranium [U]) using only 20 μL of human serum. Methods: Serum samples were digested with nitric acid and hydrochloric acid (ratio 1:1, v/v) and analysed by inductively coupled plasma–mass spectrometry (ICP-MS). Seronorm®, a human-derived serum control material was used as quality control samples. Results: The coefficient of variations for both intra- and inter-day precisions were consistently <15% for all elements. The validated method was later tested on 30 human serum samples to evaluate its applicability. Conclusion: We have successfully developed and validated a precise and accurate analytical method for determining 25 trace elements requiring very low volume of human serum.
KW - Acid digestion
KW - ICP-MS
KW - Serum
KW - Trace element
UR - http://www.scopus.com/inward/record.url?scp=85074113456&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85074113456&partnerID=8YFLogxK
U2 - 10.1016/j.plabm.2019.e00142
DO - 10.1016/j.plabm.2019.e00142
M3 - Article
AN - SCOPUS:85074113456
VL - 18
JO - Practical Laboratory Medicine
JF - Practical Laboratory Medicine
SN - 2352-5517
M1 - e00142
ER -