Simplified protocol of gel-based multiple locus variable number of tandem repeats analysis (MLVA) for Staphylococcus aureus typing

Hui Min Neoh, Choi Ying Yieng, Raja Mohd Fadhil B. Raja Abd Rahman, Salasawati Hussin, A. Rahman A. Jamal

Research output: Contribution to journalComment/debate

1 Citation (Scopus)

Abstract

Staphylococcus aureus is commonly isolated in hospital wards and easily transmitted via human-to-human contact. SmaI macro-restriction of S. aureus genomic DNA followed by pulsed-field gel electrophoresis (PFGE) typing has been reported to be the gold standard for S. aureus typing and for investigating the possibility of outbreaks in hospitals.1 However, PFGE typing requires specific equipment, technical skills, is time consuming and expensive. Sabat et al. established the gel-based multiple locus variable number of tandem repeats analysis (MLVA) for S. aureus typing and Roussel et al, reported the efficiency of this approach, showing that strain clustering is concordant to PFGE typing.2,3 The MLVA method involves PCR amplification of five variable number of tandem repeats (VNTR) loci specific for S. aureus, namely sdr, clfA, clfB, ssp, and spa in a multiplex-PCR mixture, separation of the amplified bands on an agarose gel, and comparison of the band patterns between strains to identify clusters or clones.2,3 Indeed, the VNTR loci have been used previously to differentiate S. aureus from other bacteria.2

Original languageEnglish
Pages (from-to)98-100
Number of pages3
JournalGERMS
Volume7
Issue number2
DOIs
Publication statusPublished - 2017

Fingerprint

Minisatellite Repeats
Staphylococcus aureus
Gels
Pulsed Field Gel Electrophoresis
Multiplex Polymerase Chain Reaction
Sepharose
Disease Outbreaks
Cluster Analysis
Equipment and Supplies
Polymerase Chain Reaction
DNA

Keywords

  • Multiple locus variable number of tandem repeats analysis (MLVA)
  • Pulsed-field gel electrophoresis (PFGE) typing
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Epidemiology
  • Immunology and Microbiology(all)
  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Simplified protocol of gel-based multiple locus variable number of tandem repeats analysis (MLVA) for Staphylococcus aureus typing. / Neoh, Hui Min; Yieng, Choi Ying; Raja Abd Rahman, Raja Mohd Fadhil B.; Hussin, Salasawati; A. Jamal, A. Rahman.

In: GERMS, Vol. 7, No. 2, 2017, p. 98-100.

Research output: Contribution to journalComment/debate

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