Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia

Chong Seng Shueh, Vasanthakumari Neela, Salasawati Hussin, Rukman Awang Hamat

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We developed a time-saving and cost-efficient Pulsed Field Gel Electrophoresis (PFGE) method for the typing of Stenotrophomonas maltophilia by modifying the conventional procedures. Our modifications related to the cell suspension preparation, lysis of bacterial cells in plugs, washing steps, and consumption of restriction enzyme. Although few rapid PFGE protocols on Gram-negative bacteria are available, the use of comparatively large amounts of costly reagents prompted us to look for other alternative. Hence, by considering the speed, simplicity, and relatively low cost, the modified protocol may be of more practical value than other established protocols in investigating S. maltophilia nosocomial outbreaks.

Original languageEnglish
Pages (from-to)141-143
Number of pages3
JournalJournal of Microbiological Methods
Volume94
Issue number2
DOIs
Publication statusPublished - Aug 2013

Fingerprint

Stenotrophomonas maltophilia
Pulsed Field Gel Electrophoresis
Costs and Cost Analysis
Gram-Negative Bacteria
Disease Outbreaks
Suspensions
Enzymes

Keywords

  • Cost efficient
  • Pulsed Field Gel Electrophoresis (PFGE)
  • Stenotrophomonas maltophilia
  • Time-saving

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Cite this

Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia. / Shueh, Chong Seng; Neela, Vasanthakumari; Hussin, Salasawati; Hamat, Rukman Awang.

In: Journal of Microbiological Methods, Vol. 94, No. 2, 08.2013, p. 141-143.

Research output: Contribution to journalArticle

Shueh, Chong Seng ; Neela, Vasanthakumari ; Hussin, Salasawati ; Hamat, Rukman Awang. / Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia. In: Journal of Microbiological Methods. 2013 ; Vol. 94, No. 2. pp. 141-143.
@article{15c5845a820740418f9b8177a13ecea2,
title = "Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia",
abstract = "We developed a time-saving and cost-efficient Pulsed Field Gel Electrophoresis (PFGE) method for the typing of Stenotrophomonas maltophilia by modifying the conventional procedures. Our modifications related to the cell suspension preparation, lysis of bacterial cells in plugs, washing steps, and consumption of restriction enzyme. Although few rapid PFGE protocols on Gram-negative bacteria are available, the use of comparatively large amounts of costly reagents prompted us to look for other alternative. Hence, by considering the speed, simplicity, and relatively low cost, the modified protocol may be of more practical value than other established protocols in investigating S. maltophilia nosocomial outbreaks.",
keywords = "Cost efficient, Pulsed Field Gel Electrophoresis (PFGE), Stenotrophomonas maltophilia, Time-saving",
author = "Shueh, {Chong Seng} and Vasanthakumari Neela and Salasawati Hussin and Hamat, {Rukman Awang}",
year = "2013",
month = "8",
doi = "10.1016/j.mimet.2013.06.001",
language = "English",
volume = "94",
pages = "141--143",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia

AU - Shueh, Chong Seng

AU - Neela, Vasanthakumari

AU - Hussin, Salasawati

AU - Hamat, Rukman Awang

PY - 2013/8

Y1 - 2013/8

N2 - We developed a time-saving and cost-efficient Pulsed Field Gel Electrophoresis (PFGE) method for the typing of Stenotrophomonas maltophilia by modifying the conventional procedures. Our modifications related to the cell suspension preparation, lysis of bacterial cells in plugs, washing steps, and consumption of restriction enzyme. Although few rapid PFGE protocols on Gram-negative bacteria are available, the use of comparatively large amounts of costly reagents prompted us to look for other alternative. Hence, by considering the speed, simplicity, and relatively low cost, the modified protocol may be of more practical value than other established protocols in investigating S. maltophilia nosocomial outbreaks.

AB - We developed a time-saving and cost-efficient Pulsed Field Gel Electrophoresis (PFGE) method for the typing of Stenotrophomonas maltophilia by modifying the conventional procedures. Our modifications related to the cell suspension preparation, lysis of bacterial cells in plugs, washing steps, and consumption of restriction enzyme. Although few rapid PFGE protocols on Gram-negative bacteria are available, the use of comparatively large amounts of costly reagents prompted us to look for other alternative. Hence, by considering the speed, simplicity, and relatively low cost, the modified protocol may be of more practical value than other established protocols in investigating S. maltophilia nosocomial outbreaks.

KW - Cost efficient

KW - Pulsed Field Gel Electrophoresis (PFGE)

KW - Stenotrophomonas maltophilia

KW - Time-saving

UR - http://www.scopus.com/inward/record.url?scp=84879705569&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879705569&partnerID=8YFLogxK

U2 - 10.1016/j.mimet.2013.06.001

DO - 10.1016/j.mimet.2013.06.001

M3 - Article

C2 - 23756145

AN - SCOPUS:84879705569

VL - 94

SP - 141

EP - 143

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

IS - 2

ER -