Side population cells in anaplastic thyroid cancer and normal thyroid

Kamilla Mahkamova, Nani Harlina Md. Latar, Sebastian Aspinall, Annette Meeson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Comparison of studies of cells derived from normal and pathological tissues of the same organ can be fraught with difficulties, particular with cancer where a number of different diseases are considered cancer within the same tissue. In the thyroid, there are 4 main types of cancer, three of which arise from follicular epithelial cells; papillary and follicular which are classified as differentiated, and anaplastic which is classified as undifferentiated. One assay that can be utilised for isolation of cancer stem cells is the side population (SP) assay. However, SP studies have been limited in part due to lack of optimal isolation strategies and in the case of anaplastic thyroid cancer (ATC) are further compounded by lack of access to ATC tumors. We have used thyroid cell lines to determine the optimal conditions to isolate viable SP cells. We then compared SP cells and NSP cells (bulk tumour cells without the SP) of a normal thyroid cell line N-thy ori-3-1 and an anaplastic thyroid cancer cell line SW1736 and showed that both SP cell populations displayed higher levels of stem cell characteristics than the NSP. When we compared SP cells of the N-thy ori-3-1 and the SW1736, the SW1736 SP had a higher colony forming potential, expressed higher levels of stem cell markers and CXCR4 and where more migratory and invasive, invasiveness increasing in response to CXCL12. This is the first report showing functional differences between ATC SP and normal thyroid SP and could lead to the identification of new therapeutic targets to treat ATC.

Original languageEnglish
Pages (from-to)104-113
Number of pages10
JournalExperimental Cell Research
Volume374
Issue number1
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Side-Population Cells
Thyroid Neoplasms
Thyroid Gland
Population
Neoplasms
Cell Line
Stem Cells
Neoplastic Stem Cells
Anaplastic Thyroid Carcinoma
Epithelial Cells

Keywords

  • Anaplastic thyroid cancer
  • Cancer
  • Cancer biology
  • Cancer stem cells
  • Cell invasion
  • Cell migration
  • Side population
  • Thyroid
  • Thyroid cancer

ASJC Scopus subject areas

  • Cell Biology

Cite this

Side population cells in anaplastic thyroid cancer and normal thyroid. / Mahkamova, Kamilla; Md. Latar, Nani Harlina; Aspinall, Sebastian; Meeson, Annette.

In: Experimental Cell Research, Vol. 374, No. 1, 01.01.2019, p. 104-113.

Research output: Contribution to journalArticle

Mahkamova, Kamilla ; Md. Latar, Nani Harlina ; Aspinall, Sebastian ; Meeson, Annette. / Side population cells in anaplastic thyroid cancer and normal thyroid. In: Experimental Cell Research. 2019 ; Vol. 374, No. 1. pp. 104-113.
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