Short-chain fructo-oligosaccharides produced by enzymatic hydrolysis enhance the growth of probiotics isolated from cultured milk drinks

Siti Salbiyah Abdul Fattah, Farah Diba Abu Bakar, Nor Muhammad Mahadi, Mohd Anuar Jonet, Rosli Md Illias, Abdul Munir Abd. Murad

Research output: Contribution to journalArticle

Abstract

Aims: Short-chain fructo-oligosaccharides (scFOSs) are good prebiotics that enhance the growth of probiotic bacteria. The aim of this study is to determine the effect of scFOSs produced by levan hydrolysis using recombinant endolevanase from B. lehensis G1 on the growth of probiotics isolated from commercially cultured milk drinks. Methodology and results: Two probiotic bacteria, Lactobacillus casei and L. rhamnosus, were isolated from commercially cultured milk drinks. ScFOSs were produced by levan hydrolysis using recombinant endo-levanase from B. lehensis G1. The scFOS and levan (control) were added independently to the growth medium, and the growth rates of the probiotic bacteria were determined. Results showed that the growth rate of L. casei decreased in the presence of levan compared with the control medium but increased by approximately 20% when supplemented with scFOS produced by Levblg1-N28S. Similarly, the growth rate of L. rhamnosus increased by approximately 20% when supplemented with scFOS produced by Levblg1 and Levblg1-N28S. Conclusion, significance and impact of study: The scFOSs produced by the enzymatic hydrolysis of levan using a recombinant endo-levanase from B. lehensis G1 have significant potential as prebiotics because they were able to promote the growth of the probiotic bacteria.

Original languageEnglish
Pages (from-to)497-502
Number of pages6
JournalMalaysian Journal of Microbiology
Volume14
Issue numberSpecialissue6
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Probiotics
Oligosaccharides
Milk
Hydrolysis
levanase
Growth
Bacteria
Lactobacillus casei
Prebiotics
levan

Keywords

  • Fructan
  • Fructo-oligosaccharide
  • Lactobacillus
  • Prebiotic
  • Probiotic

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Short-chain fructo-oligosaccharides produced by enzymatic hydrolysis enhance the growth of probiotics isolated from cultured milk drinks. / Abdul Fattah, Siti Salbiyah; Abu Bakar, Farah Diba; Mahadi, Nor Muhammad; Jonet, Mohd Anuar; Md Illias, Rosli; Abd. Murad, Abdul Munir.

In: Malaysian Journal of Microbiology, Vol. 14, No. Specialissue6, 01.01.2018, p. 497-502.

Research output: Contribution to journalArticle

Abdul Fattah, Siti Salbiyah ; Abu Bakar, Farah Diba ; Mahadi, Nor Muhammad ; Jonet, Mohd Anuar ; Md Illias, Rosli ; Abd. Murad, Abdul Munir. / Short-chain fructo-oligosaccharides produced by enzymatic hydrolysis enhance the growth of probiotics isolated from cultured milk drinks. In: Malaysian Journal of Microbiology. 2018 ; Vol. 14, No. Specialissue6. pp. 497-502.
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