Shelf-life evaluation of Bilayered human skin equivalent, MyDerm™

Wan Tai Seet, Manira Maarof, Khairoji Khairul Anuar, Chua Kien Hui, Abdul Wahab Ahmad Irfan, Min Hwei Ng, Bin Saim Aminuddin, Bt Hj Idrus Ruszymah

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Skin plays an important role in defense against infection and other harmful biological agents. Due to its fragile structure, skin can be easily damaged by heat, chemicals, traumatic injuries and diseases. An autologous bilayered human skin equivalent, MyDerm™, was engineered to provide a living skin substitute to treat critical skin loss. However, one of the disadvantages of living skin substitute is its short shelf-life, hence limiting its distribution worldwide. The aim of this study was to evaluate the shelf-life of MyDerm™ through assessment of cell morphology, cell viability, population doubling time and functional gene expression levels before transplantation. Skin samples were digested with 0.6% Collagenase Type I followed by epithelial cells dissociation with TrypLE Select. Dermal fibroblasts and keratinocytes were culture-expanded to obtain sufficient cells for MyDerm™ construction. MyDerm™ was constructed with plasma-fibrin as temporary biomaterial and evaluated at 0, 24, 48 and 72 hours after storage at 4°C for its shelf-life determination. The morphology of skin cells derived from MyDerm™ remained unchanged across storage times. Cells harvested from MyDerm™ after storage appeared in good viability (90.5%±2.7% to 94.9%±1.6%) and had short population doubling time (58.4±8.7 to 76.9±19 hours). The modest drop in cell viability and increased in population doubling time at longer storage duration did not demonstrate a significant difference. Gene expression for CK10, CK14 and COL III were also comparable between different storage times. In conclusion, MyDerm™ can be stored in basal medium at 4°C for at least 72 hours before transplantation without compromising its functionality.

Original languageEnglish
Article numbere40978
JournalPLoS One
Volume7
Issue number8
DOIs
Publication statusPublished - 23 Aug 2012

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skin (animal)
Skin
shelf life
Artificial Skin
Gene expression
storage time
Cells
Cell Survival
cell viability
Transplantation
Population
Gene Expression
Biological Factors
Biocompatible Materials
Collagenases
cells
Fibroblasts
Fibrin
Cell culture
gene expression

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Seet, W. T., Maarof, M., Khairul Anuar, K., Kien Hui, C., Ahmad Irfan, A. W., Ng, M. H., ... Idrus Ruszymah, B. H. (2012). Shelf-life evaluation of Bilayered human skin equivalent, MyDerm™. PLoS One, 7(8), [e40978]. https://doi.org/10.1371/journal.pone.0040978

Shelf-life evaluation of Bilayered human skin equivalent, MyDerm™. / Seet, Wan Tai; Maarof, Manira; Khairul Anuar, Khairoji; Kien Hui, Chua; Ahmad Irfan, Abdul Wahab; Ng, Min Hwei; Saim Aminuddin, Bin; Idrus Ruszymah, Bt Hj.

In: PLoS One, Vol. 7, No. 8, e40978, 23.08.2012.

Research output: Contribution to journalArticle

Seet, WT, Maarof, M, Khairul Anuar, K, Kien Hui, C, Ahmad Irfan, AW, Ng, MH, Saim Aminuddin, B & Idrus Ruszymah, BH 2012, 'Shelf-life evaluation of Bilayered human skin equivalent, MyDerm™', PLoS One, vol. 7, no. 8, e40978. https://doi.org/10.1371/journal.pone.0040978
Seet, Wan Tai ; Maarof, Manira ; Khairul Anuar, Khairoji ; Kien Hui, Chua ; Ahmad Irfan, Abdul Wahab ; Ng, Min Hwei ; Saim Aminuddin, Bin ; Idrus Ruszymah, Bt Hj. / Shelf-life evaluation of Bilayered human skin equivalent, MyDerm™. In: PLoS One. 2012 ; Vol. 7, No. 8.
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