Sex determination among Elephas maximus faecal samples collected from selected sampling plots in Taman Negara National Parks, Peninsular Malaysia

Kayal Vizi Karuppannan, Khairul Amirin Mohamed, Charles Keliang, Nurul Azura Mohd Naim, Mohd Firdaus Ariff Abdul Razak, Salmah Yaakop, Jesús E. Maldonado, Badrul Munir Md. Zain

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Sex determination among wild Asian elephant (Elephas maximus) populations in Taman Negara National Parks (TNPP), Peninsular Malaysia were very crucial by using ecological techniques such as biodiversity inventories or dung count survey. With advance molecular sexing technique, the sex ratio among wild E. maximus in study area can be determined. As a first move, we conduct this particular experiment by using two Y-specific and one X-specific molecular sexing primers on 63 wild Asian elephant faecal samples together with 10 positive controls from known elephant individuals in National Elephant Conservation Centre (NECC) Kuala Gandah, Pahang, Peninsular Malaysia. DNA extraction were done by using Qiagen kits and we carried out multiplex PCR and visualized those PCR products on agarose gel before proceed with fragment analysis. Fragment analyses with 3 peaks were confirmed for male while female showed the expected 1 peak. The outcome showed, 31 samples were from female elephants while remaining 32 samples from male. This is very significant and strong outcome which proofs that molecular sexing markers can be used in non-invasive samples as well and be a good baseline data to study Asian elephant social organization in TNPP, Peninsular Malaysia.

Original languageEnglish
Title of host publication2018 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium
EditorsNoor Hayati Ahmad Rasol, Kamarulzaman Ibrahim, Siti Aishah Hasbullah, Mohammad Hafizuddin Hj. Jumali, Nazlina Ibrahim, Marlia Mohd Hanafiah, Mohd Talib Latif
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418431
DOIs
Publication statusPublished - 27 Jun 2019
Event2018 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 4 Apr 20186 Apr 2018

Publication series

NameAIP Conference Proceedings
Volume2111
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference2018 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period4/4/186/4/18

Fingerprint

national parks
Malaysia
Elephas maximus
sex determination
elephant
national park
plots
sampling
sex determination analysis
Elephantidae
gender
fragments
biological diversity
primers
kits
markers
conservation
social structure
deoxyribonucleic acid
agarose

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science
  • Physics and Astronomy(all)
  • Nature and Landscape Conservation

Cite this

Karuppannan, K. V., Mohamed, K. A., Keliang, C., Naim, N. A. M., Razak, M. F. A. A., Yaakop, S., ... Md. Zain, B. M. (2019). Sex determination among Elephas maximus faecal samples collected from selected sampling plots in Taman Negara National Parks, Peninsular Malaysia. In N. H. A. Rasol, K. Ibrahim, S. A. Hasbullah, M. H. H. Jumali, N. Ibrahim, M. M. Hanafiah, & M. T. Latif (Eds.), 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium [060012] (AIP Conference Proceedings; Vol. 2111). American Institute of Physics Inc.. https://doi.org/10.1063/1.5111274

Sex determination among Elephas maximus faecal samples collected from selected sampling plots in Taman Negara National Parks, Peninsular Malaysia. / Karuppannan, Kayal Vizi; Mohamed, Khairul Amirin; Keliang, Charles; Naim, Nurul Azura Mohd; Razak, Mohd Firdaus Ariff Abdul; Yaakop, Salmah; Maldonado, Jesús E.; Md. Zain, Badrul Munir.

2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. ed. / Noor Hayati Ahmad Rasol; Kamarulzaman Ibrahim; Siti Aishah Hasbullah; Mohammad Hafizuddin Hj. Jumali; Nazlina Ibrahim; Marlia Mohd Hanafiah; Mohd Talib Latif. American Institute of Physics Inc., 2019. 060012 (AIP Conference Proceedings; Vol. 2111).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Karuppannan, KV, Mohamed, KA, Keliang, C, Naim, NAM, Razak, MFAA, Yaakop, S, Maldonado, JE & Md. Zain, BM 2019, Sex determination among Elephas maximus faecal samples collected from selected sampling plots in Taman Negara National Parks, Peninsular Malaysia. in NHA Rasol, K Ibrahim, SA Hasbullah, MHH Jumali, N Ibrahim, MM Hanafiah & MT Latif (eds), 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium., 060012, AIP Conference Proceedings, vol. 2111, American Institute of Physics Inc., 2018 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 4/4/18. https://doi.org/10.1063/1.5111274
Karuppannan KV, Mohamed KA, Keliang C, Naim NAM, Razak MFAA, Yaakop S et al. Sex determination among Elephas maximus faecal samples collected from selected sampling plots in Taman Negara National Parks, Peninsular Malaysia. In Rasol NHA, Ibrahim K, Hasbullah SA, Jumali MHH, Ibrahim N, Hanafiah MM, Latif MT, editors, 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. American Institute of Physics Inc. 2019. 060012. (AIP Conference Proceedings). https://doi.org/10.1063/1.5111274
Karuppannan, Kayal Vizi ; Mohamed, Khairul Amirin ; Keliang, Charles ; Naim, Nurul Azura Mohd ; Razak, Mohd Firdaus Ariff Abdul ; Yaakop, Salmah ; Maldonado, Jesús E. ; Md. Zain, Badrul Munir. / Sex determination among Elephas maximus faecal samples collected from selected sampling plots in Taman Negara National Parks, Peninsular Malaysia. 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. editor / Noor Hayati Ahmad Rasol ; Kamarulzaman Ibrahim ; Siti Aishah Hasbullah ; Mohammad Hafizuddin Hj. Jumali ; Nazlina Ibrahim ; Marlia Mohd Hanafiah ; Mohd Talib Latif. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).
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