Sequential induction of marrow stromal cells by FGF2 and BMP2 improves their growth and differentiation potential in vivo

J. Kaewsrichan, P. Wongwitwichot, K. Chandarajoti, Chua Kien Hui, Ruszymah Idrus

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Repairing bone loss by autologous grafting requires that a patient's marrow stromal cells (MSCs) be collected and cultured until the number of cells is adequate for implantation. Currently used techniques allow a slow proliferation rate and produce a culture that contains only small amounts of pluripotent stem cells that will become osteoblasts in culture. Objective: To develop culture conditions that permit a rapid increase in the number of MSCs while retaining or improving their potential for complete differentiation in vivo. Results: Sequential applications of low doses of basic fibroblast growth factor 2 (FGF2) and bone morphogenetic protein 2 (BMP2) improved the growth and differentiation potential of MSCs. FGF2 also elevated sensitivity of the cells to BMP2. BMP2 increased the syntheses of alkaline phosphatase (ALP), collagen type I and bone sialoprotein, while FGF2 increased the expression of osteocalcin (OC). Full induction as determined by the formation of mineralised nodules in vitro was observed within 7 days. Seeding the induced cells onto scaffolds and then implanting them into nude mice resulted in newly formed bone 4 weeks later. The results of real-time polymerase chain reaction (PCR) and Western blotting suggested that FGF2 increased the pool of committed osteoblasts by up-regulating the Cbfa1/Runx2 gene. The later stages of bone formation seemed to be induced by Cbfa1/Runx2-downstream factors such as BMP2, ALP, collagen type I, bone sialoprotein and OC. Conclusion: The culture system that was developed increased both the proliferation of MSC and the proportion that developed into pre-osteoblasts.

Original languageEnglish
Pages (from-to)90-101
Number of pages12
JournalArchives of Oral Biology
Volume56
Issue number1
DOIs
Publication statusPublished - Jan 2011

Fingerprint

Bone Morphogenetic Protein 2
Fibroblast Growth Factor 2
Stromal Cells
Bone Marrow
Osteoblasts
Integrin-Binding Sialoprotein
Growth
Osteocalcin
Collagen Type I
Alkaline Phosphatase
Bone and Bones
Pluripotent Stem Cells
Osteogenesis
Nude Mice
Real-Time Polymerase Chain Reaction
Cell Count
Western Blotting
Genes

Keywords

  • Basic fibroblast growth factor 2 (FGF2)
  • Bone morphogenetic protein 2 (BMP2)
  • Cbfa1/Runx2
  • Ectopic bone formation
  • Marrow stromal cells (MSCs)

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Cell Biology
  • Dentistry(all)

Cite this

Sequential induction of marrow stromal cells by FGF2 and BMP2 improves their growth and differentiation potential in vivo. / Kaewsrichan, J.; Wongwitwichot, P.; Chandarajoti, K.; Kien Hui, Chua; Idrus, Ruszymah.

In: Archives of Oral Biology, Vol. 56, No. 1, 01.2011, p. 90-101.

Research output: Contribution to journalArticle

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