Sequence variation in the cytochrome oxidase subunit I and II genes of two commonly found blow fly species, Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae) in Malaysia

Siew Hwa Tan, Edah Mohd Aris, Johari Surin, Baharudin Omar, Hiromu Kurahashi, Zulqarnain Mohamed

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The mitochondiral DNA region encompassing the cytochrome oxidase subunit I (COI) and cytochrome oxidase subunit II (COII) genes of two Malaysian blow fly species, Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart) were studied. This region, which spans 2303bp and includes the COI, tRNA leucine and partial COII was sequenced from adult fly and larval specimens, and compared. Intraspecific variations were observed at 0.26% for Ch. megacephala and 0.17% for Ch. rufifacies, while sequence divergence between the two species was recorded at a minimum of 141 out of 2303 sites (6.12%). Results obtained in this study are comparable to published data, and thus support the use of DNA sequence to facilitate and complement morphology-based species identification.

Original languageEnglish
Pages (from-to)173-181
Number of pages9
JournalTropical Biomedicine
Volume26
Issue number2
Publication statusPublished - Aug 2009

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Malaysia
Electron Transport Complex IV
Diptera
Genes
Transfer RNA
Leucine
DNA

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology

Cite this

Sequence variation in the cytochrome oxidase subunit I and II genes of two commonly found blow fly species, Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart) (Diptera : Calliphoridae) in Malaysia. / Tan, Siew Hwa; Aris, Edah Mohd; Surin, Johari; Omar, Baharudin; Kurahashi, Hiromu; Mohamed, Zulqarnain.

In: Tropical Biomedicine, Vol. 26, No. 2, 08.2009, p. 173-181.

Research output: Contribution to journalArticle

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