Sequence analysis of the PIP5Klocus in Eimeria maxima provides further evidence for eimerian genome plasticity and segmental organization

B. K. Song, M. Z. Pan, Y. L. Lau, Kiew Lian Wan

Research output: Contribution to journalArticle

Abstract

Commercial flocks infected by Eimeria species parasites, including Eimeria maxima, have an increased risk of developing clinical or subclinical coccidiosis; an intestinal enteritis associated with increased mortality rates in poultry. Currently, infection control is largely based on chemotherapy or live vaccines; however, drug resistance is common and vaccines are relatively expensive. The development of new cost-effective intervention measures will benefit from unraveling the complex genetic mechanisms that underlie host-parasite interactions, including the identification and characterization of genes encoding proteins such as phosphatidylinositol 4-phosphate 5-kinase (PIP5K). We previously identified a PIP5K coding sequence within the E. maxima genome. In this study, we analyzed two bacterial artificial chromosome clones presenting a ~145- kb E. maxima (Weybridge strain) genomic region spanning the PIP5K gene locus. Sequence analysis revealed that ~95% of the simple sequence repeats detected were located within regions comparable to the previously described feature-rich segments of the Eimeria tenella genome. Comparative sequence analysis with the orthologous E. maxima (Houghton strain) region revealed a moderate level of conserved synteny. Unique segmental organizations and telomere-like repeats were also observed in both genomes. A number of incomplete transposable elements were detected and further scrutiny of these elements in both orthologous segments revealed interesting nesting events, which may play a role in facilitating genome plasticity in E. maxima. The current analysis provides more detailed information about the genome organization of E. maxima and may help to reveal genotypic differences that are important for expression of traits related to pathogenicity and virulence.

Original languageEnglish
Pages (from-to)5803-5814
Number of pages12
JournalGenetics and Molecular Research
Volume13
Issue number3
DOIs
Publication statusPublished - 29 Jul 2014

Fingerprint

Eimeria
Sequence Analysis
Organizations
Genome
Virulence
Vaccines
Eimeria tenella
Coccidiosis
Synteny
Bacterial Artificial Chromosomes
Host-Parasite Interactions
DNA Transposable Elements
Enteritis
Telomere
Poultry
Infection Control
Drug Resistance
Microsatellite Repeats
Parasites
Clone Cells

Keywords

  • Apicomplexan parasites
  • Coccidiosis
  • Comparative sequence analysis
  • PIP5K
  • Segmental organization

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Sequence analysis of the PIP5Klocus in Eimeria maxima provides further evidence for eimerian genome plasticity and segmental organization. / Song, B. K.; Pan, M. Z.; Lau, Y. L.; Wan, Kiew Lian.

In: Genetics and Molecular Research, Vol. 13, No. 3, 29.07.2014, p. 5803-5814.

Research output: Contribution to journalArticle

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