Sequence analysis of PROTEOLYSIS 6 from Solanum lycopersicum

Nur Farhana Roslan, Bee Lyn Chew, Hoe Han Goh, Nurulhikma Md Isa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The N-end rule pathway is a protein degradation pathway that relates the protein half-life with the identity of its N-Terminal residues. A destabilizing N-Terminal residues is created by enzymatic reaction or chemical modifications. This destabilized substrate will be recognized by PROTEOLYSIS 6 (PRT6) protein, which encodes an E3 ligase enzyme and resulted in substrate degradation by proteasome. PRT6 has been studied in Arabidopsis thaliana and barley but not yet been studied in fleshy fruit plants. Hence, this study was carried out in tomato that is known as the model for fleshy fruit plants. BLASTX analysis identified that Solyc09g010830 which encodes for a PRT6 gene in tomato based on its sequence similarity with PRT6 in A.Thaliana. In silico gene expression analysis shows that PRT6 gene was highly expressed in tomato fruits breaker +5. Co-expression analysis shows that PRT6 may not only involved in abiotic stresses but also in biotic stresses. The objective is to analyze the sequence and characterize PRT6 gene in tomato.

Original languageEnglish
Title of host publication2017 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1940
ISBN (Electronic)9780735416321
DOIs
Publication statusPublished - 4 Apr 2018
Event2017 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 12 Jul 201713 Jul 2017

Other

Other2017 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period12/7/1713/7/17

Fingerprint

tomatoes
fruits
genes
proteins
barley
degradation
circuit breakers
gene expression
half life
enzymes

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Roslan, N. F., Chew, B. L., Goh, H. H., & Md Isa, N. (2018). Sequence analysis of PROTEOLYSIS 6 from Solanum lycopersicum. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium (Vol. 1940). [020062] American Institute of Physics Inc.. https://doi.org/10.1063/1.5027977

Sequence analysis of PROTEOLYSIS 6 from Solanum lycopersicum. / Roslan, Nur Farhana; Chew, Bee Lyn; Goh, Hoe Han; Md Isa, Nurulhikma.

2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018. 020062.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Roslan, NF, Chew, BL, Goh, HH & Md Isa, N 2018, Sequence analysis of PROTEOLYSIS 6 from Solanum lycopersicum. in 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. vol. 1940, 020062, American Institute of Physics Inc., 2017 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 12/7/17. https://doi.org/10.1063/1.5027977
Roslan NF, Chew BL, Goh HH, Md Isa N. Sequence analysis of PROTEOLYSIS 6 from Solanum lycopersicum. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940. American Institute of Physics Inc. 2018. 020062 https://doi.org/10.1063/1.5027977
Roslan, Nur Farhana ; Chew, Bee Lyn ; Goh, Hoe Han ; Md Isa, Nurulhikma. / Sequence analysis of PROTEOLYSIS 6 from Solanum lycopersicum. 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018.
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