Separation and identification of eugenol in ethanol extract of cloves by reversed-phase high-performance liquid chromatography

San Myint, Wan Ramli Wan Daud, Abu Bakar Mohamad, Abdul Amir H. Kadhum

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A method has been developed for the separation and identification of eugenol in the alcohol extract of cloves directly without having to previously separate other components. Extraction of eugenol with alcohol is followed by analysis with high-performance liquid chromatography. Separation by reversed-phase chromatography on low-polarity μBondapak C18 was achieved with isocratic elution. Tentative identification is based on chromatographic appearance of a peak with retention time 5.54 min for pure standard reference eugenol. The identification of eugenol was confirmed by gas chromatography/mass spectrometry analysis.

Original languageEnglish
Pages (from-to)1231-1233
Number of pages3
JournalJournal of the American Oil Chemists' Society
Volume72
Issue number10
DOIs
Publication statusPublished - Oct 1995

Fingerprint

Syzygium
Eugenol
cloves
eugenol
High performance liquid chromatography
reversed-phase high performance liquid chromatography
Reverse-Phase Chromatography
Alcohols
Ethanol
High Pressure Liquid Chromatography
ethanol
extracts
Chromatography
Gas chromatography
Mass spectrometry
alcohols
reference standards
Gas Chromatography-Mass Spectrometry
chromatography
high performance liquid chromatography

Keywords

  • Clove alcohol extract
  • eugenol
  • optimization
  • reversed-phase HPLC

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Food Science
  • Chemical Engineering(all)
  • Organic Chemistry

Cite this

Separation and identification of eugenol in ethanol extract of cloves by reversed-phase high-performance liquid chromatography. / Myint, San; Wan Daud, Wan Ramli; Mohamad, Abu Bakar; Kadhum, Abdul Amir H.

In: Journal of the American Oil Chemists' Society, Vol. 72, No. 10, 10.1995, p. 1231-1233.

Research output: Contribution to journalArticle

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