Sensitivity of acanthamoeba cyst to antimicrobial agents

S. A. Noradilah, Mohamed Kamel Abdul Ghani, Anisah Nordin, A. R. Noraina, S. Yusof

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Introduction: Acanthamoeba is an ubiquitous free-living protozoa which causes serious ocular problems. Acanthamoeba keratitis is becoming more prevalent amongst contact lens wearers. The disease can cause loss of vision and blindness if not treated properly. The objective of this research is to study the sensitivity of six Acanthamoeba spp. isolates, of which three were from the clinical isolates (HKL 95, HTH 40 and HS 6) and the remaining three from environmental isolates (TTT 9, TL 3 and SMAL 8) to antimicrobial agents. Methods: The antimicrobial agents chosen for this purpose were polyhexamethylene biguanide (PHMB) and Chlorhexidine. Serial dilutions were perfomed forpolyhexamemylene biguanide and Chlorhexidine. Cyst suspensions from the chosen isolates were exposed to PHMB and Chlorhexidine respectively. After 48 hours incubation time at 30°C, each mixture was filtered and filtration membrane was put onto non-nutrient agar laid with Escherichia coli. The agar plates were incubated for three days at 30°C and examined daily until day 14 to detect the presence of Acanthamoeba trophozoites under the inverted microscope. The presence of trophozoites indicated me ineffectiveness of the antimicrobial agents. Results: Both of the antimicrobial agents tested were found to be effective against Acanthamoeba cysts from all die test strains. Polyhexamethylene biguanide gave a minimum cysticidal concentration (MCC) mean value of 2.848 ug/mL while Chlorhexidine showed MCC mean value at a concentration of 3.988 μg/mL. Contusion: It can be concluded that me Acanthamoeba cysts were sensitive to polyhexamethylene biguanide and Chlorhexidine.

Original languageEnglish
Pages (from-to)111-117
Number of pages7
JournalMalaysian Journal of Medicine and Health Sciences
Volume6
Issue number1
Publication statusPublished - Jan 2010

Fingerprint

Acanthamoeba
Chlorhexidine
Anti-Infective Agents
Cysts
Trophozoites
HS 6
Agar
Acanthamoeba Keratitis
Biguanides
Contusions
Contact Lenses
Blindness
Suspensions
Escherichia coli
Membranes
polyhexamethylene biguanide
Research

Keywords

  • Acanthamoeba
  • Chlorhexidine
  • Polyhexamethylene biguanide
  • Sensitivity

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Sensitivity of acanthamoeba cyst to antimicrobial agents. / Noradilah, S. A.; Abdul Ghani, Mohamed Kamel; Nordin, Anisah; Noraina, A. R.; Yusof, S.

In: Malaysian Journal of Medicine and Health Sciences, Vol. 6, No. 1, 01.2010, p. 111-117.

Research output: Contribution to journalArticle

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