Selective uptake of alpha-tocotrienol and improvement in oxidative status in rat brains following short- and long-term intake of tocotrienol rich fraction

M. Musalmah, K. S. Leow, M. T. Nursiati, I. Raja Najmi Hanis Raja, F. Fadly Syah, S. Renuka, S. M. Siti Norsyamimi, Mohamad Fairuz Yahaya, A. L. Azian

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Tocotrienol exerts neuroprotective effects resulting in an improved circulating oxidative status. However, accumulation of tocotrienol due to longterm intake may exert pro-oxidant effects. Thus the effects of short- and longterm supplementation of vitamin E tocotrienol rich fraction (TRF) on the parameters of oxidative status in rat brains were determined. Methods: Wistar rats aged 3 months were supplemented with TRF for 3 or 8 months. Control groups received equivolume of distilled water. Rats were sacrificed and brains harvested, weighed and homogenised. Supernatants were analysed for catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities, vitamin E and protein carbonyl. Results: A significant decline in the level of total vitamin E and its isomers with increasing age were found. TRF supplementation increased the level of total vitamin E with alpha-tocotrienol (ATT) being the major isomer raised. Glutathione peroxidase activity was also significantly increased in the long-term supplemented group compared to the short-term supplemented and control groups. The results also showed significantly higher superoxide dismutase activity (p<0.001), lower protein peroxidation (p<0.05) and heavier brain weights (p<0.05) in both supplemented groups but catalase activity remained unchanged. Conclusion: The study showed long- term TRF supplementation exerts positive effects on brain oxidative status.

Original languageEnglish
Pages (from-to)251-259
Number of pages9
JournalMalaysian Journal of Nutrition
Volume19
Issue number2
Publication statusPublished - 2013

Fingerprint

alpha-tocotrienol
Tocotrienols
tocotrienols
Vitamin E
uptake mechanisms
brain
vitamin E
rats
Brain
glutathione peroxidase
Glutathione Peroxidase
Catalase
Superoxide Dismutase
isomers
catalase
superoxide dismutase
neuroprotective effect
Control Groups
peroxidation
Neuroprotective Agents

Keywords

  • Antioxidant
  • Brain
  • Long-term supplementation
  • Oxidative stress
  • Tocotrienol rich fraction (TRF)

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Food Science

Cite this

Musalmah, M., Leow, K. S., Nursiati, M. T., Raja Najmi Hanis Raja, I., Fadly Syah, F., Renuka, S., ... Azian, A. L. (2013). Selective uptake of alpha-tocotrienol and improvement in oxidative status in rat brains following short- and long-term intake of tocotrienol rich fraction. Malaysian Journal of Nutrition, 19(2), 251-259.

Selective uptake of alpha-tocotrienol and improvement in oxidative status in rat brains following short- and long-term intake of tocotrienol rich fraction. / Musalmah, M.; Leow, K. S.; Nursiati, M. T.; Raja Najmi Hanis Raja, I.; Fadly Syah, F.; Renuka, S.; Siti Norsyamimi, S. M.; Yahaya, Mohamad Fairuz; Azian, A. L.

In: Malaysian Journal of Nutrition, Vol. 19, No. 2, 2013, p. 251-259.

Research output: Contribution to journalArticle

Musalmah, M, Leow, KS, Nursiati, MT, Raja Najmi Hanis Raja, I, Fadly Syah, F, Renuka, S, Siti Norsyamimi, SM, Yahaya, MF & Azian, AL 2013, 'Selective uptake of alpha-tocotrienol and improvement in oxidative status in rat brains following short- and long-term intake of tocotrienol rich fraction', Malaysian Journal of Nutrition, vol. 19, no. 2, pp. 251-259.
Musalmah, M. ; Leow, K. S. ; Nursiati, M. T. ; Raja Najmi Hanis Raja, I. ; Fadly Syah, F. ; Renuka, S. ; Siti Norsyamimi, S. M. ; Yahaya, Mohamad Fairuz ; Azian, A. L. / Selective uptake of alpha-tocotrienol and improvement in oxidative status in rat brains following short- and long-term intake of tocotrienol rich fraction. In: Malaysian Journal of Nutrition. 2013 ; Vol. 19, No. 2. pp. 251-259.
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AU - Musalmah, M.

AU - Leow, K. S.

AU - Nursiati, M. T.

AU - Raja Najmi Hanis Raja, I.

AU - Fadly Syah, F.

AU - Renuka, S.

AU - Siti Norsyamimi, S. M.

AU - Yahaya, Mohamad Fairuz

AU - Azian, A. L.

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AB - Tocotrienol exerts neuroprotective effects resulting in an improved circulating oxidative status. However, accumulation of tocotrienol due to longterm intake may exert pro-oxidant effects. Thus the effects of short- and longterm supplementation of vitamin E tocotrienol rich fraction (TRF) on the parameters of oxidative status in rat brains were determined. Methods: Wistar rats aged 3 months were supplemented with TRF for 3 or 8 months. Control groups received equivolume of distilled water. Rats were sacrificed and brains harvested, weighed and homogenised. Supernatants were analysed for catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities, vitamin E and protein carbonyl. Results: A significant decline in the level of total vitamin E and its isomers with increasing age were found. TRF supplementation increased the level of total vitamin E with alpha-tocotrienol (ATT) being the major isomer raised. Glutathione peroxidase activity was also significantly increased in the long-term supplemented group compared to the short-term supplemented and control groups. The results also showed significantly higher superoxide dismutase activity (p<0.001), lower protein peroxidation (p<0.05) and heavier brain weights (p<0.05) in both supplemented groups but catalase activity remained unchanged. Conclusion: The study showed long- term TRF supplementation exerts positive effects on brain oxidative status.

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