Selective loss of B-cell phenotype in lymphocyte predominant Hodgkin lymphoma

S. Tedoldi, A. Mottok, J. Ying, J. C. Paterson, Y. Cui, F. Facchetti, J. H J M Van Krieken, M. Ponzoni, S. Özkal, Noraidah Masir, Y. Natkunam, S. A. Pileri, M. L. Hansmann, D. Y. Mason, Q. Tao, T. Marafioti

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The neoplastic Reed-Sternberg cells characteristic of classical Hodgkin's lymphoma (cHL) are of B-cell origin but they almost always show striking loss of a range of B-cell-associated molecules. In contrast, the neoplastic cells found in lymphocyte predominant Hodgkin's lymphoma (LPHL) (L&H cells) are traditionally thought of as possessing the full repertoire of features associated with germinal centre B cells (eg BCL-6 expression, 'ongoing' Ig gene mutation). In the present paper, we report an extensive phenotypic analysis of L&H cells which revealed down-regulation of a number of markers associated with the B-cell lineage (eg CD19, CD37) and with the germinal centre maturation stage (eg PAG, LCK). The promoter methylation status of three of these down-regulated genes (CD10, CD19, and LCK) was further studied in microdissected L&H cells, and this revealed that their promoters were unmethylated. In contrast, these genes showed promoter methylation in cell lines derived from CHL. Further investigation of the mechanisms responsible for the deregulation of these molecules in L&H cells may provide new insights into the genetic abnormalities underlying LPHL.

Original languageEnglish
Pages (from-to)429-440
Number of pages12
JournalJournal of Pathology
Volume213
Issue number4
DOIs
Publication statusPublished - Dec 2007
Externally publishedYes

Fingerprint

Hodgkin Disease
B-Lymphocytes
Lymphocytes
Phenotype
Germinal Center
Methylation
Reed-Sternberg Cells
Immunoglobulin Genes
Cell Lineage
Genes
Down-Regulation
Cell Line
Mutation

Keywords

  • Immunohistochemistry
  • LPHL
  • Methylation
  • Microdissection

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Tedoldi, S., Mottok, A., Ying, J., Paterson, J. C., Cui, Y., Facchetti, F., ... Marafioti, T. (2007). Selective loss of B-cell phenotype in lymphocyte predominant Hodgkin lymphoma. Journal of Pathology, 213(4), 429-440. https://doi.org/10.1002/path.2242

Selective loss of B-cell phenotype in lymphocyte predominant Hodgkin lymphoma. / Tedoldi, S.; Mottok, A.; Ying, J.; Paterson, J. C.; Cui, Y.; Facchetti, F.; Van Krieken, J. H J M; Ponzoni, M.; Özkal, S.; Masir, Noraidah; Natkunam, Y.; Pileri, S. A.; Hansmann, M. L.; Mason, D. Y.; Tao, Q.; Marafioti, T.

In: Journal of Pathology, Vol. 213, No. 4, 12.2007, p. 429-440.

Research output: Contribution to journalArticle

Tedoldi, S, Mottok, A, Ying, J, Paterson, JC, Cui, Y, Facchetti, F, Van Krieken, JHJM, Ponzoni, M, Özkal, S, Masir, N, Natkunam, Y, Pileri, SA, Hansmann, ML, Mason, DY, Tao, Q & Marafioti, T 2007, 'Selective loss of B-cell phenotype in lymphocyte predominant Hodgkin lymphoma', Journal of Pathology, vol. 213, no. 4, pp. 429-440. https://doi.org/10.1002/path.2242
Tedoldi S, Mottok A, Ying J, Paterson JC, Cui Y, Facchetti F et al. Selective loss of B-cell phenotype in lymphocyte predominant Hodgkin lymphoma. Journal of Pathology. 2007 Dec;213(4):429-440. https://doi.org/10.1002/path.2242
Tedoldi, S. ; Mottok, A. ; Ying, J. ; Paterson, J. C. ; Cui, Y. ; Facchetti, F. ; Van Krieken, J. H J M ; Ponzoni, M. ; Özkal, S. ; Masir, Noraidah ; Natkunam, Y. ; Pileri, S. A. ; Hansmann, M. L. ; Mason, D. Y. ; Tao, Q. ; Marafioti, T. / Selective loss of B-cell phenotype in lymphocyte predominant Hodgkin lymphoma. In: Journal of Pathology. 2007 ; Vol. 213, No. 4. pp. 429-440.
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