Penindasan terpilih gen-gen Staphylococcus aureus rintang metisilin yang dirawat dengan ekstrak metanol Melastoma malabathricum

Translated title of the contribution: Selective inhibition of genes in Methicillin Resistant Staphylococcus aureus (MRSA) treated with Melastoma malabathricum methanol extract

Zulaikah Mohamed, Nazlina Ibrahim, Ismail Ahmad

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Methanol extract of Melastoma malabathricum leaves inhibited the growth of Staphylococcus aureus and six clinical isolates of Methicilin Resistant Stapyhlococcus aureus (MRSA 1-6). The minimum inhibitory concentration (MIC) of test substance was 1.565mg/ml and the minimum bacteriocidal concentration (MBC) was 3.125 mg/ml. The methanol extract suppressed RNA synthesis at 10 mg/ml as shown by RNA profile which was devoid of three bands compared to the control. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis using seven primer pairs was only successful in amplifying four cDNA amplicons. The failure to amplify three cDNA amplicons for three primer pairs corresponding to gyrA, femA and nuc genes, implied the possibility of suppression of the corresponding mRNA. Electrophoretic separation of endogenous and exogenuos bacterial proteins showed that three and five protein, respectively were not expressed. One endogenous and three exogenous proteins were over-expressed in treated MRSA compared with untreated control. The results of the molecular and proteomic analyses are in agreement and based on primers being used, methanol extract of M. malabathricum leaves possibly inhibits MRSA growth through inhibition of DNA synthesis, peptidoglycan production and nuclease production.

Original languageUndefined/Unknown
Pages (from-to)107-113
Number of pages7
JournalSains Malaysiana
Volume37
Issue number1
Publication statusPublished - Mar 2008

Fingerprint

Melastoma malabathricum
methanol
extracts
RNA
bacterial proteins
synthesis
genes
nucleases
peptidoglycans
minimum inhibitory concentration
proteomics
growth retardation
leaves
Staphylococcus aureus
proteins
reverse transcriptase polymerase chain reaction
DNA
methicillin-resistant Staphylococcus aureus
testing

Keywords

  • Gene expression
  • Melastoma malabathricum
  • Methicillin resistant Staphylococcus aureus
  • Protein production

ASJC Scopus subject areas

  • General

Cite this

Penindasan terpilih gen-gen Staphylococcus aureus rintang metisilin yang dirawat dengan ekstrak metanol Melastoma malabathricum. / Mohamed, Zulaikah; Ibrahim, Nazlina; Ahmad, Ismail.

In: Sains Malaysiana, Vol. 37, No. 1, 03.2008, p. 107-113.

Research output: Contribution to journalArticle

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