Selection of potential petroleum hydrocarbon-degrading bacteria

A. Hamzah, O. Omar, Aidil Abdul Hamid, Sahidan Senafi, Wan Mohtar Wan Yusoff, N. Hambali, F. Rahim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Effluent from oil-refinery wastewater treatment plants may contain various types of contaminants, particularly hydrocarbons. The effluent quality has to meet the regulatory limit prior to discharge. Therefore the contaminants need to be treated to avoid noncompliance as well as to prevent environmental pollution, particularly of surface water and groundwater. A search for local isolates of microorganisms that are capable of degrading these hydrocarbon contaminants was carried out because bioremediation is less toxic to the environment. In this study, 129 local bacterial isolates have been isolated from the sludge and wastewater oil refinery treatment plant. Mineral salt medium (MSM) with addition of 0.01% nystatin and 1% of crude oil was used as selection medium to screen for hydrocarbon degraders. The bacteria were characterized morphologically, physiologically, and biochemically as suggested by Bergey's Manual Determinative of Bacteriology. For primary screening, the isolates were selected based on the ability to grow at different temperatures (37d̀C, 40d̀C, and 45d̀C) and they are classified into class 1, 2 or 3 based on the time for the colony to appear on the agar plate at 24, 48 and 72 hours, respectively. Fourteen isolates were able to grow in 24 hours at 37d̀C and these isolates were further characterized by growing in MSM in the present of 1% Khefji-Dubai Sour crude oil, Angsi light, Bunga Kekwa heavy and Dulang medium crude oils. From the growth profile result, five isolates were selected since they showed higher number of cells than the others. These isolates were identified as Pseudomonas multiresinivorans (3M16), Pseudomonas aeruginosa sp1 (6M1), Acinetobacter baumannii (3M17), Pseudomonas aeruginosa sp2 (6M2) and Rhodococcus rubber (10M2) by PCR and 16S rDNA sequences. These isolates were also able to degrade Khefji Dubai Sour crude oil with percentage of oil degradation between 20% to 40% after 7 days incubation at 37d̀C.

Original languageEnglish
Title of host publicationBattelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007
Pages315-320
Number of pages6
Volume1
Publication statusPublished - 2007
Event9th International In Situ and On-Site Bioremediation Symposium 2007 - Baltimore, MD
Duration: 7 May 200710 May 2007

Other

Other9th International In Situ and On-Site Bioremediation Symposium 2007
CityBaltimore, MD
Period7/5/0710/5/07

Fingerprint

Petroleum
petroleum hydrocarbon
Hydrocarbons
crude oil
Bacteria
Crude oil
Oils
bacterium
Impurities
hydrocarbon
Minerals
pollutant
Effluents
Bacteriology
Salts
bacteriology
effluent
salt
Nystatin
Bioremediation

ASJC Scopus subject areas

  • Ecological Modelling
  • Environmental Engineering
  • Bioengineering
  • Waste Management and Disposal

Cite this

Hamzah, A., Omar, O., Abdul Hamid, A., Senafi, S., Wan Yusoff, W. M., Hambali, N., & Rahim, F. (2007). Selection of potential petroleum hydrocarbon-degrading bacteria. In Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007 (Vol. 1, pp. 315-320)

Selection of potential petroleum hydrocarbon-degrading bacteria. / Hamzah, A.; Omar, O.; Abdul Hamid, Aidil; Senafi, Sahidan; Wan Yusoff, Wan Mohtar; Hambali, N.; Rahim, F.

Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007. Vol. 1 2007. p. 315-320.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hamzah, A, Omar, O, Abdul Hamid, A, Senafi, S, Wan Yusoff, WM, Hambali, N & Rahim, F 2007, Selection of potential petroleum hydrocarbon-degrading bacteria. in Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007. vol. 1, pp. 315-320, 9th International In Situ and On-Site Bioremediation Symposium 2007, Baltimore, MD, 7/5/07.
Hamzah A, Omar O, Abdul Hamid A, Senafi S, Wan Yusoff WM, Hambali N et al. Selection of potential petroleum hydrocarbon-degrading bacteria. In Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007. Vol. 1. 2007. p. 315-320
Hamzah, A. ; Omar, O. ; Abdul Hamid, Aidil ; Senafi, Sahidan ; Wan Yusoff, Wan Mohtar ; Hambali, N. ; Rahim, F. / Selection of potential petroleum hydrocarbon-degrading bacteria. Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007. Vol. 1 2007. pp. 315-320
@inproceedings{e7ac9d8f798e4bc7ba7747ae977cc00d,
title = "Selection of potential petroleum hydrocarbon-degrading bacteria",
abstract = "Effluent from oil-refinery wastewater treatment plants may contain various types of contaminants, particularly hydrocarbons. The effluent quality has to meet the regulatory limit prior to discharge. Therefore the contaminants need to be treated to avoid noncompliance as well as to prevent environmental pollution, particularly of surface water and groundwater. A search for local isolates of microorganisms that are capable of degrading these hydrocarbon contaminants was carried out because bioremediation is less toxic to the environment. In this study, 129 local bacterial isolates have been isolated from the sludge and wastewater oil refinery treatment plant. Mineral salt medium (MSM) with addition of 0.01{\%} nystatin and 1{\%} of crude oil was used as selection medium to screen for hydrocarbon degraders. The bacteria were characterized morphologically, physiologically, and biochemically as suggested by Bergey's Manual Determinative of Bacteriology. For primary screening, the isolates were selected based on the ability to grow at different temperatures (37d̀C, 40d̀C, and 45d̀C) and they are classified into class 1, 2 or 3 based on the time for the colony to appear on the agar plate at 24, 48 and 72 hours, respectively. Fourteen isolates were able to grow in 24 hours at 37d̀C and these isolates were further characterized by growing in MSM in the present of 1{\%} Khefji-Dubai Sour crude oil, Angsi light, Bunga Kekwa heavy and Dulang medium crude oils. From the growth profile result, five isolates were selected since they showed higher number of cells than the others. These isolates were identified as Pseudomonas multiresinivorans (3M16), Pseudomonas aeruginosa sp1 (6M1), Acinetobacter baumannii (3M17), Pseudomonas aeruginosa sp2 (6M2) and Rhodococcus rubber (10M2) by PCR and 16S rDNA sequences. These isolates were also able to degrade Khefji Dubai Sour crude oil with percentage of oil degradation between 20{\%} to 40{\%} after 7 days incubation at 37d̀C.",
author = "A. Hamzah and O. Omar and {Abdul Hamid}, Aidil and Sahidan Senafi and {Wan Yusoff}, {Wan Mohtar} and N. Hambali and F. Rahim",
year = "2007",
language = "English",
isbn = "9781604239485",
volume = "1",
pages = "315--320",
booktitle = "Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007",

}

TY - GEN

T1 - Selection of potential petroleum hydrocarbon-degrading bacteria

AU - Hamzah, A.

AU - Omar, O.

AU - Abdul Hamid, Aidil

AU - Senafi, Sahidan

AU - Wan Yusoff, Wan Mohtar

AU - Hambali, N.

AU - Rahim, F.

PY - 2007

Y1 - 2007

N2 - Effluent from oil-refinery wastewater treatment plants may contain various types of contaminants, particularly hydrocarbons. The effluent quality has to meet the regulatory limit prior to discharge. Therefore the contaminants need to be treated to avoid noncompliance as well as to prevent environmental pollution, particularly of surface water and groundwater. A search for local isolates of microorganisms that are capable of degrading these hydrocarbon contaminants was carried out because bioremediation is less toxic to the environment. In this study, 129 local bacterial isolates have been isolated from the sludge and wastewater oil refinery treatment plant. Mineral salt medium (MSM) with addition of 0.01% nystatin and 1% of crude oil was used as selection medium to screen for hydrocarbon degraders. The bacteria were characterized morphologically, physiologically, and biochemically as suggested by Bergey's Manual Determinative of Bacteriology. For primary screening, the isolates were selected based on the ability to grow at different temperatures (37d̀C, 40d̀C, and 45d̀C) and they are classified into class 1, 2 or 3 based on the time for the colony to appear on the agar plate at 24, 48 and 72 hours, respectively. Fourteen isolates were able to grow in 24 hours at 37d̀C and these isolates were further characterized by growing in MSM in the present of 1% Khefji-Dubai Sour crude oil, Angsi light, Bunga Kekwa heavy and Dulang medium crude oils. From the growth profile result, five isolates were selected since they showed higher number of cells than the others. These isolates were identified as Pseudomonas multiresinivorans (3M16), Pseudomonas aeruginosa sp1 (6M1), Acinetobacter baumannii (3M17), Pseudomonas aeruginosa sp2 (6M2) and Rhodococcus rubber (10M2) by PCR and 16S rDNA sequences. These isolates were also able to degrade Khefji Dubai Sour crude oil with percentage of oil degradation between 20% to 40% after 7 days incubation at 37d̀C.

AB - Effluent from oil-refinery wastewater treatment plants may contain various types of contaminants, particularly hydrocarbons. The effluent quality has to meet the regulatory limit prior to discharge. Therefore the contaminants need to be treated to avoid noncompliance as well as to prevent environmental pollution, particularly of surface water and groundwater. A search for local isolates of microorganisms that are capable of degrading these hydrocarbon contaminants was carried out because bioremediation is less toxic to the environment. In this study, 129 local bacterial isolates have been isolated from the sludge and wastewater oil refinery treatment plant. Mineral salt medium (MSM) with addition of 0.01% nystatin and 1% of crude oil was used as selection medium to screen for hydrocarbon degraders. The bacteria were characterized morphologically, physiologically, and biochemically as suggested by Bergey's Manual Determinative of Bacteriology. For primary screening, the isolates were selected based on the ability to grow at different temperatures (37d̀C, 40d̀C, and 45d̀C) and they are classified into class 1, 2 or 3 based on the time for the colony to appear on the agar plate at 24, 48 and 72 hours, respectively. Fourteen isolates were able to grow in 24 hours at 37d̀C and these isolates were further characterized by growing in MSM in the present of 1% Khefji-Dubai Sour crude oil, Angsi light, Bunga Kekwa heavy and Dulang medium crude oils. From the growth profile result, five isolates were selected since they showed higher number of cells than the others. These isolates were identified as Pseudomonas multiresinivorans (3M16), Pseudomonas aeruginosa sp1 (6M1), Acinetobacter baumannii (3M17), Pseudomonas aeruginosa sp2 (6M2) and Rhodococcus rubber (10M2) by PCR and 16S rDNA sequences. These isolates were also able to degrade Khefji Dubai Sour crude oil with percentage of oil degradation between 20% to 40% after 7 days incubation at 37d̀C.

UR - http://www.scopus.com/inward/record.url?scp=58449098136&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58449098136&partnerID=8YFLogxK

M3 - Conference contribution

SN - 9781604239485

VL - 1

SP - 315

EP - 320

BT - Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007

ER -