Seizure attainment and mortality of mice in kainate- induced status epilepticus

Nurulumi Ahmad, Hui Yin Yow, Mohd Makmor Bakry

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: To evaluate two different methods of intraperitoneal (IP) administration and range of doses to establish optimal dose to induce status epilepticus (SE) in mice, the incidence of achieving stage 4 (S4) or above SE and mortality in kainic acid (KA)-induced SE. Methods: KA was administered as bolus with dose range between 20 and 40 mg, and by intermittent injection of 25 mg and followed by 5 mg doses with 30-minute interval between doses. Each dose was tested on 3 mice. Following KA injections, mice were monitored continuously for the onset of SE, extent of seizure activity and mortality. Results: All ICR mice displayed comparable seizures. Within eight minutes of the injection, mice were inactive accompanied by staring behaviour. This behaviour was followed by jerking, tail arching, head nodding, forelimb clonus, rearing, falling and generalised tonic-clonic seizure. Bolus dose of KA 30 mg/kg and above is sufficient to induce SE at S4 or above. Out of 18 mice, 8 reached S4 or above SE. For the 8 mice, 4 died from SE within 4 days depending on the dose given. Conclusion: In mice, bolus dose of KA 30 mg/kg is sufficient to induce significant SE characteristics. Survival of the mice with S4 or above SE is up to 4 days depending on dose of KA given.

Original languageEnglish
Pages (from-to)145-147
Number of pages3
JournalInternational Journal of Pharmacy and Pharmaceutical Sciences
Volume5
Issue numberSUPPL 3
Publication statusPublished - 2013

Fingerprint

Status Epilepticus
Kainic Acid
Seizures
Mortality
Injections
Inbred ICR Mouse
Forelimb
Tail
Incidence

Keywords

  • Kainic acid
  • Seizure attainment
  • Status epilepticus

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Seizure attainment and mortality of mice in kainate- induced status epilepticus. / Ahmad, Nurulumi; Yow, Hui Yin; Makmor Bakry, Mohd.

In: International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 5, No. SUPPL 3, 2013, p. 145-147.

Research output: Contribution to journalArticle

@article{b20be90febe34f9c898ff19e8eeb591a,
title = "Seizure attainment and mortality of mice in kainate- induced status epilepticus",
abstract = "Objective: To evaluate two different methods of intraperitoneal (IP) administration and range of doses to establish optimal dose to induce status epilepticus (SE) in mice, the incidence of achieving stage 4 (S4) or above SE and mortality in kainic acid (KA)-induced SE. Methods: KA was administered as bolus with dose range between 20 and 40 mg, and by intermittent injection of 25 mg and followed by 5 mg doses with 30-minute interval between doses. Each dose was tested on 3 mice. Following KA injections, mice were monitored continuously for the onset of SE, extent of seizure activity and mortality. Results: All ICR mice displayed comparable seizures. Within eight minutes of the injection, mice were inactive accompanied by staring behaviour. This behaviour was followed by jerking, tail arching, head nodding, forelimb clonus, rearing, falling and generalised tonic-clonic seizure. Bolus dose of KA 30 mg/kg and above is sufficient to induce SE at S4 or above. Out of 18 mice, 8 reached S4 or above SE. For the 8 mice, 4 died from SE within 4 days depending on the dose given. Conclusion: In mice, bolus dose of KA 30 mg/kg is sufficient to induce significant SE characteristics. Survival of the mice with S4 or above SE is up to 4 days depending on dose of KA given.",
keywords = "Kainic acid, Seizure attainment, Status epilepticus",
author = "Nurulumi Ahmad and Yow, {Hui Yin} and {Makmor Bakry}, Mohd",
year = "2013",
language = "English",
volume = "5",
pages = "145--147",
journal = "International Journal of Pharmacy and Pharmaceutical Sciences",
issn = "0975-1491",
publisher = "IJPPS",
number = "SUPPL 3",

}

TY - JOUR

T1 - Seizure attainment and mortality of mice in kainate- induced status epilepticus

AU - Ahmad, Nurulumi

AU - Yow, Hui Yin

AU - Makmor Bakry, Mohd

PY - 2013

Y1 - 2013

N2 - Objective: To evaluate two different methods of intraperitoneal (IP) administration and range of doses to establish optimal dose to induce status epilepticus (SE) in mice, the incidence of achieving stage 4 (S4) or above SE and mortality in kainic acid (KA)-induced SE. Methods: KA was administered as bolus with dose range between 20 and 40 mg, and by intermittent injection of 25 mg and followed by 5 mg doses with 30-minute interval between doses. Each dose was tested on 3 mice. Following KA injections, mice were monitored continuously for the onset of SE, extent of seizure activity and mortality. Results: All ICR mice displayed comparable seizures. Within eight minutes of the injection, mice were inactive accompanied by staring behaviour. This behaviour was followed by jerking, tail arching, head nodding, forelimb clonus, rearing, falling and generalised tonic-clonic seizure. Bolus dose of KA 30 mg/kg and above is sufficient to induce SE at S4 or above. Out of 18 mice, 8 reached S4 or above SE. For the 8 mice, 4 died from SE within 4 days depending on the dose given. Conclusion: In mice, bolus dose of KA 30 mg/kg is sufficient to induce significant SE characteristics. Survival of the mice with S4 or above SE is up to 4 days depending on dose of KA given.

AB - Objective: To evaluate two different methods of intraperitoneal (IP) administration and range of doses to establish optimal dose to induce status epilepticus (SE) in mice, the incidence of achieving stage 4 (S4) or above SE and mortality in kainic acid (KA)-induced SE. Methods: KA was administered as bolus with dose range between 20 and 40 mg, and by intermittent injection of 25 mg and followed by 5 mg doses with 30-minute interval between doses. Each dose was tested on 3 mice. Following KA injections, mice were monitored continuously for the onset of SE, extent of seizure activity and mortality. Results: All ICR mice displayed comparable seizures. Within eight minutes of the injection, mice were inactive accompanied by staring behaviour. This behaviour was followed by jerking, tail arching, head nodding, forelimb clonus, rearing, falling and generalised tonic-clonic seizure. Bolus dose of KA 30 mg/kg and above is sufficient to induce SE at S4 or above. Out of 18 mice, 8 reached S4 or above SE. For the 8 mice, 4 died from SE within 4 days depending on the dose given. Conclusion: In mice, bolus dose of KA 30 mg/kg is sufficient to induce significant SE characteristics. Survival of the mice with S4 or above SE is up to 4 days depending on dose of KA given.

KW - Kainic acid

KW - Seizure attainment

KW - Status epilepticus

UR - http://www.scopus.com/inward/record.url?scp=84882758222&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84882758222&partnerID=8YFLogxK

M3 - Article

VL - 5

SP - 145

EP - 147

JO - International Journal of Pharmacy and Pharmaceutical Sciences

JF - International Journal of Pharmacy and Pharmaceutical Sciences

SN - 0975-1491

IS - SUPPL 3

ER -