Seeds of giant dodder (Cuscuta reflexa) as a function of extract procedure and solvent nature

Shazia Noureen, Sobia Noreen, Shazia Akram Ghumman, Fozia Batool, Mahira Arshad, Fozia Noreen, Umair Ishtiaq, Bukhari Syed Nasir Abbas

Research output: Contribution to journalArticle

Abstract

Seeds of a renowned medicinal plant, giant dodder (Cuscuta reflexa), were assessed to appraise the effect of solvent and extraction technique variation on antioxidants potential. Dodder seed, also called cuscuta seed, has been considered superb tonic in traditional herbal medication for eyes, liver, spleen and kidney. Results show that selected solvent and procedure plays a key role in the composition and activity of extractable material. Three extraction procedures Orbital shaker, Decoction and Ultrasonic assisted extraction and five different solvents n-hexane, ethyl acetate, 100% methanol, 80% methanol and 60% methanol were used to get optimized conditions. Total phenolic and flavonoids content were found maximum in the extracts of aqueous organic system containing 80% methanol in Ultrasonic assisted extraction method but in case of tannins ethyl acetate and Orbital shaker extraction was found more suitable partner. Antioxidant estimation assays showed a little bit variation as DPPH and ABTS exhibited maximum inhibition in 80% methanol and Ultrasonic assisted extraction but 100% methanol was found better for FRAP assay. Decoction results were mostly in between the both Orbital shaker and Ultrasonic assisted extraction. Overall results indicate that coexistence of polar solvents and Ultrasonic assisted extraction gives a better choice for extractability of potent antioxidants from seeds. HPLC analysis confirmed presence of valuable phenolic acids. Pearson's correlation coefficient reveals a significant relationship between extracted components and antioxidant capacity P < 0.05 or 0.01.

Original languageEnglish
Pages (from-to)653-662
Number of pages10
JournalNotulae Botanicae Horti Agrobotanici Cluj-Napoca
Volume46
Issue number2
DOIs
Publication statusPublished - 1 Jan 2018
Externally publishedYes

Fingerprint

Cuscuta reflexa
methanol
extracts
ultrasonics
seeds
antioxidants
Cuscuta
assays
phenolic acids
hexane
drug therapy
tannins
medicinal plants
spleen
flavonoids
eyes
kidneys
liver

Keywords

  • Antioxidants
  • Decoction
  • Orbital shaking
  • Phenolic acids
  • Ultrasonic assisted extraction

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Horticulture

Cite this

Seeds of giant dodder (Cuscuta reflexa) as a function of extract procedure and solvent nature. / Noureen, Shazia; Noreen, Sobia; Ghumman, Shazia Akram; Batool, Fozia; Arshad, Mahira; Noreen, Fozia; Ishtiaq, Umair; Syed Nasir Abbas, Bukhari.

In: Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Vol. 46, No. 2, 01.01.2018, p. 653-662.

Research output: Contribution to journalArticle

Noureen, Shazia ; Noreen, Sobia ; Ghumman, Shazia Akram ; Batool, Fozia ; Arshad, Mahira ; Noreen, Fozia ; Ishtiaq, Umair ; Syed Nasir Abbas, Bukhari. / Seeds of giant dodder (Cuscuta reflexa) as a function of extract procedure and solvent nature. In: Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2018 ; Vol. 46, No. 2. pp. 653-662.
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