Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes

How Lie Ling, Zaidah Rahmat, Farah Diba Abu Bakar, Abdul Munir Abd. Murad, Rosli Md Illias

Research output: Contribution to journalArticle

Abstract

Bacillus lehensis G1 is an alkaliphilic bacterium that is capable of surviving in environments up to pH 11. Secretome related to bacterial acclimation in alkaline environment has been less studied compared to cytoplasmic and membrane proteome. The aim of this study was to gain better understanding of bacterial acclimation to alkaline media through analyzing extracellular proteins of B. lehensis. The pH range for B. lehensis growth was conducted, and two-dimensional electrophoresis and MALDI-TOF/TOF MS analysis were conducted to characterize changes in protein profiling in B. lehensis cultured at pH 8 and pH 11 when compared with those cultured at pH 10 (optimal growth pH). B. lehensis could grow well at pH ranging from 8 to 11 in which the bacteria showed to posses thinner flagella at pH 11. Proteomic analyses demonstrated that five proteins were up-regulated and 13 proteins were down-regulated at pH 8, whereas at pH 11, 14 proteins were up-regulated and 8 were down-regulated. Majority of the differentially expressed proteins were involved in the cell wall, main glycolytic pathways, the metabolism of amino acids and related molecules and some proteins of unknown function. A total of 40 differentially expressed protein spots corresponding to 33 proteins were identified; including GlcNAc-binding protein A, chitinase, endopeptidase lytE, flagellar hook-associated proteins and enolase. These proteins may play important roles in acclimation to alkaline media via reallocation of cell wall structure and changes to cell surface glycolytic enzymes, amino acid metabolism, flagellar hook-associated proteins and chaperones to sustain life under pH-stressed conditions.

Original languageEnglish
Pages (from-to)46-54
Number of pages9
JournalMicrobiological Research
Volume215
DOIs
Publication statusPublished - 1 Oct 2018

Fingerprint

Bacillus
Bacteria
Proteins
Acclimatization
Cell Wall
Amino Acids
Chitinases
Endopeptidases
Flagella
Phosphopyruvate Hydratase
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Staphylococcal Protein A
Proteome
Growth
Proteomics
Electrophoresis
Carrier Proteins
Cell Membrane
Enzymes

Keywords

  • 2-DE
  • Alkaliphile
  • Bacillus lehensis
  • Secretome

ASJC Scopus subject areas

  • Microbiology

Cite this

Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes. / Ling, How Lie; Rahmat, Zaidah; Abu Bakar, Farah Diba; Abd. Murad, Abdul Munir; Illias, Rosli Md.

In: Microbiological Research, Vol. 215, 01.10.2018, p. 46-54.

Research output: Contribution to journalArticle

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