Secretion of wound healing mediators by single and bi-layer skin substitutes

Manira Maarof, Jia Xian Law, Shiplu Roy Chowdhury, Khairul Anuar Khairoji, Aminuddin Bin Saim, Ruszymah Idrus

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Limitations of current treatments for skin loss caused by major injuries leads to the use of skin substitutes. It is assumed that secretion of wound healing mediators by these skin substitutes plays a role in treating skin loss. In our previous study, single layer keratinocytes (SK), single layer fibroblast (SF) and bilayer (BL; containing keratinocytes and fibroblasts layers) skin substitutes were fabricated using fibrin that had shown potential to heal wounds in preclinical studies. This study aimed to quantify the secretion of wound healing mediators, and compare between single and bi-layer skin substitutes. Skin samples were digested to harvest fibroblasts and keratinocytes, and expanded to obtain sufficient cells for the construction of skin substitutes. Acellular fibrin (AF) construct was used as control. Substitutes i.e. AF, SK, SF and BL were cultured for 2 days, and culture supernatant was collected to analyze secretion of wound healing mediators via multiplex ELISA. Among 19 wound healing mediators tested, BL substitute secreted significantly higher amounts of CXCL1 and GCSF compared to SF and AF substitute but this was not significant with respect to SK substitute. The BL substitute also secreted significantly higher amounts of CXCL5 and IL-6 compared to other substitutes. In contrast, the SK substitute secreted significantly higher amounts of VCAM-1 compared to other substitutes. However, all three skin substitutes also secreted CCL2, CCL5, CCL11, GM-CSF, IL8, IL-1α, TNF-α, ICAM-1, FGF-β, TGF-β, HGF, VEGF-α and PDGF-BB factors, but no significant difference was seen. Secretion of these mediators after transplantation may play a significant role in promoting wound healing process for the treatment of skin loss.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalCytotechnology
DOIs
Publication statusAccepted/In press - 14 Jan 2016

Fingerprint

Artificial Skin
Keratinocytes
Wound Healing
Fibroblasts
Skin
Fibrin
Vascular Cell Adhesion Molecule-1
Wounds and Injuries
Intercellular Adhesion Molecule-1
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-8
Interleukin-1
Vascular Endothelial Growth Factor A
Interleukin-6
Transplantation
Enzyme-Linked Immunosorbent Assay
Therapeutics

Keywords

  • Bi-layer skin substitute
  • Fibrin
  • Fibroblasts
  • Keratinocytes
  • Tissue engineering

ASJC Scopus subject areas

  • Cell Biology
  • Biotechnology
  • Biomedical Engineering
  • Bioengineering
  • Clinical Biochemistry

Cite this

Secretion of wound healing mediators by single and bi-layer skin substitutes. / Maarof, Manira; Law, Jia Xian; Chowdhury, Shiplu Roy; Khairoji, Khairul Anuar; Saim, Aminuddin Bin; Idrus, Ruszymah.

In: Cytotechnology, 14.01.2016, p. 1-12.

Research output: Contribution to journalArticle

Maarof, Manira ; Law, Jia Xian ; Chowdhury, Shiplu Roy ; Khairoji, Khairul Anuar ; Saim, Aminuddin Bin ; Idrus, Ruszymah. / Secretion of wound healing mediators by single and bi-layer skin substitutes. In: Cytotechnology. 2016 ; pp. 1-12.
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