Screening of transgenic tobacco for resistance against cucumber mosaic virus

Kalaivani K. Nadarajah, N. M. Hanafi, S. L. Tan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Coat protein (CP), Movement Protein (MP) and Overlapping (OVG) genes were isolated from a Malaysian Cucumber mosaic virus (CMV) isolate via RT-PCR and transformed into Nicotiana tabacum through Agrobacterium tumefaciens-mediadted transformation. Out of the thirty six independently transformed lines developed from the three different genes and the mutants of MP and OVG. five lines were tested for resistance against CMV by challenge inoculations using three different concentrations (1:10, 3:10 and 5:10) of CMV macerated in 0.1 M sodium phosphate buffer (pH 7.0). The transgenic lines exhibiting complete resistance remained symptomless even when re-inoculated with 1:10 concentration of virus. The level of viral RNA accumulation in inoculated leaves was significantly (at least 2-3 times) lower compared to the control untransformed plants. The upper leaves which were analysed for systemic spread of the infection had much lower levels of viral RNA accumulation compared to the inoculated leaves. Amongst the three genes and two mutant lines that were generated in this study, we found that the CP and MP genes were able to provide a better level of resistance to the plants compared to the overlapping gene.

Original languageEnglish
Pages (from-to)42-52
Number of pages11
JournalPlant Pathology Journal
Volume8
Issue number2
Publication statusPublished - 2009

Fingerprint

Cucumber mosaic virus
tobacco
genetically modified organisms
screening
coat proteins
genes
RNA
leaves
mutants
sodium phosphate
proteins
Agrobacterium radiobacter
Nicotiana tabacum
buffers
reverse transcriptase polymerase chain reaction
viruses
infection

Keywords

  • CMV
  • Coat protein
  • Disease resistance
  • Movement protein
  • Nicotiana tabacum
  • Overlapping gene
  • Transgenic lines

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Screening of transgenic tobacco for resistance against cucumber mosaic virus. / K. Nadarajah, Kalaivani; Hanafi, N. M.; Tan, S. L.

In: Plant Pathology Journal, Vol. 8, No. 2, 2009, p. 42-52.

Research output: Contribution to journalArticle

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