Screening of rickettsia sp. In ticks (acari: Ixodidae) collected from small mammals in three recreational forests in Selangor, Malaysia

Siti Nabilah Ishak, Lim Fang Shiang, Khoo Jing Jing, Farah Shafawati Mohd Taib

Research output: Contribution to journalArticle

Abstract

Rickettsia is a gram-negative, non-motile, and obligate intracellular bacterium that usually associated with arthropods vectors such as ticks, fleas, lice and mites. These bacteria have the ability to cause diseases in humans, however, in Malaysia, knowledge on the prevalence and distribution of these bacteria mainly focused on humans and information of these bacteria in small mammal hosts is limited. Thus, this study aims to investigate the presence of Rickettsia sp. in the DNA of tick’s samples collected from small mammals in three different recreational forests in Selangor, Malaysia. Sampling was conducted in which 200 cage traps were set up randomly along streams and forest trails for five nights. A total of 106 fully engorged and adult ticks were collected from 23 individuals of seven small mammals host species. All samples were tested for Rickettsia bacteria based on polymerase chain reaction (PCR) using partial 17-kDa antigen gene. The PCR results obtained from this study showed no infestation of Rickettsia sp. in all tick samples. Our findings revealed that none of the tick samples from these forests’ sites were infected with Rickettsia pathogen, however, more intense and extensive surveillance for Rickettsia sp. from other tick species is still necessary for greater geographical areas across Malaysia.

Original languageEnglish
Pages (from-to)116-125
Number of pages10
JournalSerangga
Volume24
Issue number1
Publication statusPublished - 1 Jan 2019

Fingerprint

Rickettsia
tick
Ixodidae
small mammal
small mammals
Malaysia
ticks
Acari
screening
bacterium
bacteria
polymerase chain reaction
sampling
louse
flea
lice
Siphonaptera
man-made trails
antigen
human diseases

Keywords

  • Ixodes granulatus
  • Small mammals
  • Tick-borne diseases
  • Ticks

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

Cite this

Screening of rickettsia sp. In ticks (acari : Ixodidae) collected from small mammals in three recreational forests in Selangor, Malaysia. / Ishak, Siti Nabilah; Shiang, Lim Fang; Jing, Khoo Jing; Mohd Taib, Farah Shafawati.

In: Serangga, Vol. 24, No. 1, 01.01.2019, p. 116-125.

Research output: Contribution to journalArticle

@article{cda20059f8aa483b92bbbf378f8afe60,
title = "Screening of rickettsia sp. In ticks (acari: Ixodidae) collected from small mammals in three recreational forests in Selangor, Malaysia",
abstract = "Rickettsia is a gram-negative, non-motile, and obligate intracellular bacterium that usually associated with arthropods vectors such as ticks, fleas, lice and mites. These bacteria have the ability to cause diseases in humans, however, in Malaysia, knowledge on the prevalence and distribution of these bacteria mainly focused on humans and information of these bacteria in small mammal hosts is limited. Thus, this study aims to investigate the presence of Rickettsia sp. in the DNA of tick’s samples collected from small mammals in three different recreational forests in Selangor, Malaysia. Sampling was conducted in which 200 cage traps were set up randomly along streams and forest trails for five nights. A total of 106 fully engorged and adult ticks were collected from 23 individuals of seven small mammals host species. All samples were tested for Rickettsia bacteria based on polymerase chain reaction (PCR) using partial 17-kDa antigen gene. The PCR results obtained from this study showed no infestation of Rickettsia sp. in all tick samples. Our findings revealed that none of the tick samples from these forests’ sites were infected with Rickettsia pathogen, however, more intense and extensive surveillance for Rickettsia sp. from other tick species is still necessary for greater geographical areas across Malaysia.",
keywords = "Ixodes granulatus, Small mammals, Tick-borne diseases, Ticks",
author = "Ishak, {Siti Nabilah} and Shiang, {Lim Fang} and Jing, {Khoo Jing} and {Mohd Taib}, {Farah Shafawati}",
year = "2019",
month = "1",
day = "1",
language = "English",
volume = "24",
pages = "116--125",
journal = "Serangga",
issn = "1394-5130",
publisher = "Universiti Kebangsaan Malaysia",
number = "1",

}

TY - JOUR

T1 - Screening of rickettsia sp. In ticks (acari

T2 - Ixodidae) collected from small mammals in three recreational forests in Selangor, Malaysia

AU - Ishak, Siti Nabilah

AU - Shiang, Lim Fang

AU - Jing, Khoo Jing

AU - Mohd Taib, Farah Shafawati

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Rickettsia is a gram-negative, non-motile, and obligate intracellular bacterium that usually associated with arthropods vectors such as ticks, fleas, lice and mites. These bacteria have the ability to cause diseases in humans, however, in Malaysia, knowledge on the prevalence and distribution of these bacteria mainly focused on humans and information of these bacteria in small mammal hosts is limited. Thus, this study aims to investigate the presence of Rickettsia sp. in the DNA of tick’s samples collected from small mammals in three different recreational forests in Selangor, Malaysia. Sampling was conducted in which 200 cage traps were set up randomly along streams and forest trails for five nights. A total of 106 fully engorged and adult ticks were collected from 23 individuals of seven small mammals host species. All samples were tested for Rickettsia bacteria based on polymerase chain reaction (PCR) using partial 17-kDa antigen gene. The PCR results obtained from this study showed no infestation of Rickettsia sp. in all tick samples. Our findings revealed that none of the tick samples from these forests’ sites were infected with Rickettsia pathogen, however, more intense and extensive surveillance for Rickettsia sp. from other tick species is still necessary for greater geographical areas across Malaysia.

AB - Rickettsia is a gram-negative, non-motile, and obligate intracellular bacterium that usually associated with arthropods vectors such as ticks, fleas, lice and mites. These bacteria have the ability to cause diseases in humans, however, in Malaysia, knowledge on the prevalence and distribution of these bacteria mainly focused on humans and information of these bacteria in small mammal hosts is limited. Thus, this study aims to investigate the presence of Rickettsia sp. in the DNA of tick’s samples collected from small mammals in three different recreational forests in Selangor, Malaysia. Sampling was conducted in which 200 cage traps were set up randomly along streams and forest trails for five nights. A total of 106 fully engorged and adult ticks were collected from 23 individuals of seven small mammals host species. All samples were tested for Rickettsia bacteria based on polymerase chain reaction (PCR) using partial 17-kDa antigen gene. The PCR results obtained from this study showed no infestation of Rickettsia sp. in all tick samples. Our findings revealed that none of the tick samples from these forests’ sites were infected with Rickettsia pathogen, however, more intense and extensive surveillance for Rickettsia sp. from other tick species is still necessary for greater geographical areas across Malaysia.

KW - Ixodes granulatus

KW - Small mammals

KW - Tick-borne diseases

KW - Ticks

UR - http://www.scopus.com/inward/record.url?scp=85070769898&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070769898&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:85070769898

VL - 24

SP - 116

EP - 125

JO - Serangga

JF - Serangga

SN - 1394-5130

IS - 1

ER -