Screening of antibacterial and anticancer activity of soft corals from Togean Islands, Indonesia

Muhammad Sulaiman Zubair, Subehan Lallo, Rusmianti, Arsa Wahyu Nugrahani, Ibrahim Jantan

Research output: Contribution to journalArticle

Abstract

Soft corals (Octocorallia, Alcyonaceae) have been reported to possess diverse biological activities and unique structural chemistry. This study aims to screen the potential antibacterial and anticancer activity of some soft corals collected from Togean Islands, Central Sulawesi, Indonesia. They were Lobophytum sp, Sarcophyton sp, Sinularia sp 1, and Sinularia sp 2. All dried coral materials were extracted for 3 x 24 h by maceration method using methanol and then evaporated by rotary evaporator to obtain viscous extracts. The determination of antibacterial activity had been performed by well agar diffusion method against Staphylococcus aureus and Escherichia coli. Meanwhile, the cytotoxic activity was performed by MTT method, followed by apoptosis annexin V-FTIC assay. Identification for the presence of terpenoids was performed by p-anisaldehyde-sulphuric acid spraying reagent on thin layer chromatography (TLC). Sinularia sp 2 extract have strongly inhibited S. aureus and E.coli with the diameter of inhibition of 15.80 mm and 15.73 mm, respectively. Moreover, Sinularia sp 2 extract possessed also cytotoxic activity against human breast adenocarcinoma (MCF-7) and colorectal carcinoma (HCT-116) with the IC50 of 46.807 and 47.186 μg/mL, respectively. Extract Sinularia sp 1 was found to have strongest cytotoxicity on human colon colorectal carcinoma (HCT-116) with the IC50 of < 1.505 μg/mL. Annexin V-FTIC assay clearly exhibited that the apoptosis mechanism is proposed by the extracts of Sinularia sp 1 and Sinularia sp 2. Terpenoids were identified on both extracts suggesting for further purification and isolation for the bioactive terpenoid compounds.

Original languageEnglish
Pages (from-to)173-178
Number of pages6
JournalIndonesian Journal of Pharmacy
Volume29
Issue number4
DOIs
Publication statusPublished - 1 Jan 2018
Externally publishedYes

Fingerprint

Anthozoa
Indonesia
Terpenes
Islands
Screening
Annexin A5
Escherichia coli
Inhibitory Concentration 50
Staphylococcus aureus
Colorectal Neoplasms
Assays
Apoptosis
Thin layer chromatography
Evaporators
Spraying
Cytotoxicity
Thin Layer Chromatography
Bioactivity
Human Activities
Agar

Keywords

  • Anti-cancer
  • Antibacterial
  • Softcorals
  • Togean Islands

ASJC Scopus subject areas

  • Pharmacy
  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

Screening of antibacterial and anticancer activity of soft corals from Togean Islands, Indonesia. / Zubair, Muhammad Sulaiman; Lallo, Subehan; Rusmianti; Nugrahani, Arsa Wahyu; Jantan, Ibrahim.

In: Indonesian Journal of Pharmacy, Vol. 29, No. 4, 01.01.2018, p. 173-178.

Research output: Contribution to journalArticle

Zubair, Muhammad Sulaiman ; Lallo, Subehan ; Rusmianti ; Nugrahani, Arsa Wahyu ; Jantan, Ibrahim. / Screening of antibacterial and anticancer activity of soft corals from Togean Islands, Indonesia. In: Indonesian Journal of Pharmacy. 2018 ; Vol. 29, No. 4. pp. 173-178.
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