Screening for New Delhi metallo-β-lactamase-1 in enterobacteriaceae

Is there a role for the modified hodge test?

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1 Citation (Scopus)

Abstract

Objective: The New Delhi metallo-β-lactamase-1 (NDM-1) enzyme is a plasmid-encoded enzyme that inactivates carbapenem antibiotics. This study aims to ascertain if the modified Hodge test (MHT) has a role in screening for NDM-1 in Enterobacteriaceae with reduced carbapenem susceptibility. Methods: Over a period of one year, all Enterobacteriaceae isolates from all clinical specimens with reduced susceptibility to at least one carbapenem were subjected to MHT and conventional polymerase chain reaction (PCR) detection of the NDM-1 gene. Results: A total of 13,098 Enterobacteriaceae isolates were screened and 63 (0.48%) had reduced susceptibility to at least one carbapenem. Out of the 63 isolates, 45 (71.4%) were MHT-positive. The NDM- 1 gene was detected in 18 of the 63 isolates (28.6%). All 18 PCR-positive isolates were also MHT-positive. Thus, the sensitivity and specificity of the MHT in detecting NDM-1 in Enterobacteriaceae with reduced carbapenem susceptibility are 100% and 40%, respectively. Conclusion: The MHT is a useful test to screen for the presence of NDM-1 in Enterobacteriaceae with reduced carbapenem susceptibility. However, due to its rather low specificity, all MHT-positive isolates should be subjected to alternative tests (e.g. PCR) for confirmation, especially if other types of carbapenemases (e.g. KPC) are prevalent.

Original languageEnglish
Pages (from-to)1340-1343
Number of pages4
JournalPakistan Journal of Medical Sciences
Volume31
Issue number6
DOIs
Publication statusPublished - 2015

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Carbapenems
Enterobacteriaceae
Polymerase Chain Reaction
Enzymes
Genes
Plasmids
Sensitivity and Specificity

Keywords

  • Enterobacteriaceae
  • Metallo-β-lactamase-1
  • MHT
  • Modified Hodge test
  • NDM-1

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{5eab7f271cdb4160899ae1acc8d8a6df,
title = "Screening for New Delhi metallo-β-lactamase-1 in enterobacteriaceae: Is there a role for the modified hodge test?",
abstract = "Objective: The New Delhi metallo-β-lactamase-1 (NDM-1) enzyme is a plasmid-encoded enzyme that inactivates carbapenem antibiotics. This study aims to ascertain if the modified Hodge test (MHT) has a role in screening for NDM-1 in Enterobacteriaceae with reduced carbapenem susceptibility. Methods: Over a period of one year, all Enterobacteriaceae isolates from all clinical specimens with reduced susceptibility to at least one carbapenem were subjected to MHT and conventional polymerase chain reaction (PCR) detection of the NDM-1 gene. Results: A total of 13,098 Enterobacteriaceae isolates were screened and 63 (0.48{\%}) had reduced susceptibility to at least one carbapenem. Out of the 63 isolates, 45 (71.4{\%}) were MHT-positive. The NDM- 1 gene was detected in 18 of the 63 isolates (28.6{\%}). All 18 PCR-positive isolates were also MHT-positive. Thus, the sensitivity and specificity of the MHT in detecting NDM-1 in Enterobacteriaceae with reduced carbapenem susceptibility are 100{\%} and 40{\%}, respectively. Conclusion: The MHT is a useful test to screen for the presence of NDM-1 in Enterobacteriaceae with reduced carbapenem susceptibility. However, due to its rather low specificity, all MHT-positive isolates should be subjected to alternative tests (e.g. PCR) for confirmation, especially if other types of carbapenemases (e.g. KPC) are prevalent.",
keywords = "Enterobacteriaceae, Metallo-β-lactamase-1, MHT, Modified Hodge test, NDM-1",
author = "Abidin, {Nor Zanariah Zainol} and Anita Sulong and Alfizah Hanafiah and Ding, {Chuan Hun} and {Abdul Samat}, {Muttaqillah Najihan} and Rahman, {Md. Mostafizur}",
year = "2015",
doi = "10.12669/pjms.316.8159",
language = "English",
volume = "31",
pages = "1340--1343",
journal = "Pakistan Journal of Medical Sciences",
issn = "1682-024X",
publisher = "Professional Medical Publications",
number = "6",

}

TY - JOUR

T1 - Screening for New Delhi metallo-β-lactamase-1 in enterobacteriaceae

T2 - Is there a role for the modified hodge test?

AU - Abidin, Nor Zanariah Zainol

AU - Sulong, Anita

AU - Hanafiah, Alfizah

AU - Ding, Chuan Hun

AU - Abdul Samat, Muttaqillah Najihan

AU - Rahman, Md. Mostafizur

PY - 2015

Y1 - 2015

N2 - Objective: The New Delhi metallo-β-lactamase-1 (NDM-1) enzyme is a plasmid-encoded enzyme that inactivates carbapenem antibiotics. This study aims to ascertain if the modified Hodge test (MHT) has a role in screening for NDM-1 in Enterobacteriaceae with reduced carbapenem susceptibility. Methods: Over a period of one year, all Enterobacteriaceae isolates from all clinical specimens with reduced susceptibility to at least one carbapenem were subjected to MHT and conventional polymerase chain reaction (PCR) detection of the NDM-1 gene. Results: A total of 13,098 Enterobacteriaceae isolates were screened and 63 (0.48%) had reduced susceptibility to at least one carbapenem. Out of the 63 isolates, 45 (71.4%) were MHT-positive. The NDM- 1 gene was detected in 18 of the 63 isolates (28.6%). All 18 PCR-positive isolates were also MHT-positive. Thus, the sensitivity and specificity of the MHT in detecting NDM-1 in Enterobacteriaceae with reduced carbapenem susceptibility are 100% and 40%, respectively. Conclusion: The MHT is a useful test to screen for the presence of NDM-1 in Enterobacteriaceae with reduced carbapenem susceptibility. However, due to its rather low specificity, all MHT-positive isolates should be subjected to alternative tests (e.g. PCR) for confirmation, especially if other types of carbapenemases (e.g. KPC) are prevalent.

AB - Objective: The New Delhi metallo-β-lactamase-1 (NDM-1) enzyme is a plasmid-encoded enzyme that inactivates carbapenem antibiotics. This study aims to ascertain if the modified Hodge test (MHT) has a role in screening for NDM-1 in Enterobacteriaceae with reduced carbapenem susceptibility. Methods: Over a period of one year, all Enterobacteriaceae isolates from all clinical specimens with reduced susceptibility to at least one carbapenem were subjected to MHT and conventional polymerase chain reaction (PCR) detection of the NDM-1 gene. Results: A total of 13,098 Enterobacteriaceae isolates were screened and 63 (0.48%) had reduced susceptibility to at least one carbapenem. Out of the 63 isolates, 45 (71.4%) were MHT-positive. The NDM- 1 gene was detected in 18 of the 63 isolates (28.6%). All 18 PCR-positive isolates were also MHT-positive. Thus, the sensitivity and specificity of the MHT in detecting NDM-1 in Enterobacteriaceae with reduced carbapenem susceptibility are 100% and 40%, respectively. Conclusion: The MHT is a useful test to screen for the presence of NDM-1 in Enterobacteriaceae with reduced carbapenem susceptibility. However, due to its rather low specificity, all MHT-positive isolates should be subjected to alternative tests (e.g. PCR) for confirmation, especially if other types of carbapenemases (e.g. KPC) are prevalent.

KW - Enterobacteriaceae

KW - Metallo-β-lactamase-1

KW - MHT

KW - Modified Hodge test

KW - NDM-1

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U2 - 10.12669/pjms.316.8159

DO - 10.12669/pjms.316.8159

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JO - Pakistan Journal of Medical Sciences

JF - Pakistan Journal of Medical Sciences

SN - 1682-024X

IS - 6

ER -