Screening for antiviral activity of sweet lemon grass (Cymbopogon nardus (L.) Rendle) fractions

M. N Nurul Aini, M. I. Said, Nazlina Ibrahim, M. N. Hanina, Ishak Ahmad

Research output: Contribution to journalArticle

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Abstract

A study was carried out to test the cytotoxicity and antiviral effects of sweet lemon grass (Cymbopogon nardus (L.) Rendle) fractions. A total of 51 fractions were obtained after two sequential runs of flash chromatography. Further fractionation on selected fractions by preparative Thin Layer Chromatography (TLC) resulted in 40 sub fractions. The cytotoxicity towards Vero cells of the different fractions ranged from highly toxic (N3 and N29; 30 μg mL-1) to considerably non toxic (N16, N17 and N19; 1 mg mL-1). For antiviral activity test the cells were inoculated with 10 μL measles virus 1000 TCID50 and treated with 1.0 LC50. 0.1 LC50 or 0.01 LC50 concentrations of each fraction. Most fractions protected cell death due to measles inoculation when compared with the untreated controls, but were less effective when compared to the control guanidine hydrochloride treatment of 0.1 mg mL-1. Treatment of Vero cells with fractions at 0.1 LC50 gave higher cell survival when compared to the other concentrations, which were 1.0 LC50 or 0.01 LC50. At 0.1 LC50, the C. nardus fractions were most effective when cells (C) were treated with the fractions (F) before being inoculated with the virus (V), which was treatment {(C+F) + V}. Using this method, more fractions with higher efficacy (+++) was obtained when compared with the methods {(C+V) + F} or {C + (V+F)}. Similar, treatment of cell culture with sub fractions before virus inoculations showed better antiviral effect that treatment after viral inoculations. This suggests that fractions and sub fractions were more effective in protecting cells against entry of virus particles into inoculated cells than other phases in the viral replication.

Original languageEnglish
Pages (from-to)507-510
Number of pages4
JournalJournal of Biological Sciences
Volume6
Issue number3
DOIs
Publication statusPublished - May 2006

Fingerprint

Cymbopogon
Antiviral Agents
Vero Cells
Poisons
Viruses
Measles virus
Guanidine
Measles
Thin Layer Chromatography
Virion
Chromatography
Cell Survival
Cell Death
Cell Culture Techniques

Keywords

  • Antiviral activity
  • Cymbopogon nardus
  • Cytotoxicity
  • Measles virus

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine

Cite this

Screening for antiviral activity of sweet lemon grass (Cymbopogon nardus (L.) Rendle) fractions. / Aini, M. N Nurul; Said, M. I.; Ibrahim, Nazlina; Hanina, M. N.; Ahmad, Ishak.

In: Journal of Biological Sciences, Vol. 6, No. 3, 05.2006, p. 507-510.

Research output: Contribution to journalArticle

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