Screening and optimization of medium composition for mannanase production by Aspergillus terreus SUK-1 in solid state fermentation using statistical experimental methods

Jahwarhar Izuan Abd Rashid, Noraini Samat, Wan Mohtar Wan Yusoff

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this study, two statistical experimental methods including Plackett Burman design and Response Surface Methodology (RSM) were employed to optimize the medium composition for mannanase production by Aspergillus terreus SUK-1, a locally isolated fungus using Palm Kernel Cake (PKC) as the sole carbon source. A total of 19 media were screened in order to determine the optimum media for mannanase production using Plackett-Burman design. Soya bean, proteose peptone, urea and NH4NO3 were identified as the supplement to give positive effect on mannanase production by A. terreus SUK-1. Proteose peptone was more significant in terms of its effect on mannanase production compared to urea. The optimum of the medium composition was predicted when the concentrations of proteose peptone and urea were set at 0.5 and 1% (g g-1), respectively. The optimum mannanase activity was closed to the predicted value 30.24±1.32-32.60 IU g-1 when the optimum medium composition was employed. Under the optimized medium (0.5% (g g-1) of proteose peptone and 1% (g g-1) of urea), Mannanase production was 3-fold higher than the activity obtained under original medium (9.97±1.34 IU g-1).

Original languageEnglish
Pages (from-to)242-255
Number of pages14
JournalResearch Journal of Microbiology
Volume7
Issue number5
DOIs
Publication statusPublished - 2012

Fingerprint

Aspergillus terreus
beta-mannosidase
solid state fermentation
Aspergillus
proteose peptones
Fermentation
Urea
screening
urea
methodology
palm kernel cake
Fungi
Carbon
response surface methodology
proteose-peptone
beans
fungi
carbon

Keywords

  • Aspergilus terreus SUK-1
  • Mannose enzyme
  • Plackett burman design
  • Response surface methodology solid state fermentation

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Infectious Diseases
  • Microbiology

Cite this

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abstract = "In this study, two statistical experimental methods including Plackett Burman design and Response Surface Methodology (RSM) were employed to optimize the medium composition for mannanase production by Aspergillus terreus SUK-1, a locally isolated fungus using Palm Kernel Cake (PKC) as the sole carbon source. A total of 19 media were screened in order to determine the optimum media for mannanase production using Plackett-Burman design. Soya bean, proteose peptone, urea and NH4NO3 were identified as the supplement to give positive effect on mannanase production by A. terreus SUK-1. Proteose peptone was more significant in terms of its effect on mannanase production compared to urea. The optimum of the medium composition was predicted when the concentrations of proteose peptone and urea were set at 0.5 and 1{\%} (g g-1), respectively. The optimum mannanase activity was closed to the predicted value 30.24±1.32-32.60 IU g-1 when the optimum medium composition was employed. Under the optimized medium (0.5{\%} (g g-1) of proteose peptone and 1{\%} (g g-1) of urea), Mannanase production was 3-fold higher than the activity obtained under original medium (9.97±1.34 IU g-1).",
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PY - 2012

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