Screen-printed histamine biosensors fabricated from the entrapment of diamine oxidase in a photocured poly(HEMA) film

Ching Mai Keow, Fatimah Abu Bakar, Abu Bakar Salleh, Yook Heng Lee, Rahman Wagiran, Shafiquzzaman Siddiquee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A three-electrode system amperometric biosensor that consisted of screen-printed carbon paste working electrode (SPE), a commercial platinum rod counter electrode vs Ag/AgCl reference electrode was developed for rapid determination of histamine in prawns. The biosensor was designed from entrapping diamine oxidase (DAO) enzyme in a poly(2-hydroxyethyl methacrylate) (photoHEMA) film prepared via a simple and one-step direct photocuring process on a carbon paste screen-printed electrode (SPE). The photoHEMA film exhibited water absorption of 34.14% after four hours exposure to water and no leaching of DAO was observed from the hydrogel film. The histamine biosensor showed response time of < 50 s with a linear response range from 0 to 60 ppm histamine (R 2 of 0.9946). The sensitivity of the biosensor was of 5.56 nAppm -1, with a limit detection of 0.65 ppm histamine. The performance of the fabricated biosensor in the analysis of histamine in tiger prawn (Penaeus monodon) samples was comparable to high performance liquid chromatography (HPLC). The three-electrode system was then converted to an all-screen-printed histamine biosensor by printing onto a polyester substrate with carbon paste to form the working and counter electrodes and Ag/AgCl paste as the reference electrode. The performance of all-screen-printed histamine biosensor was evaluated using potassium hexacyanoferrate (III) as a mediator deposited electrochemically on the carbon-paste SPE. The presence of this mediator demonstrated improvement to the response of the all-screen- printed histamine biosensor.

Original languageEnglish
Pages (from-to)4702-4715
Number of pages14
JournalInternational Journal of Electrochemical Science
Volume7
Issue number5
Publication statusPublished - 2012

Fingerprint

Amine Oxidase (Copper-Containing)
Biosensors
Histamine
Electrodes
Ointments
Carbon
poly(2-hydroxyethyl methacrylate)-polyamine graft copolymer
Oxidoreductases
Polyesters
High performance liquid chromatography
Water absorption
Platinum
Hydrogels
Leaching
Potassium
Printing
Enzymes

Keywords

  • Diamine oxidase
  • Histamine
  • Photocurable methacrylate film
  • Screen-printed amperometric biosensor
  • Tiger prawn

ASJC Scopus subject areas

  • Electrochemistry

Cite this

Screen-printed histamine biosensors fabricated from the entrapment of diamine oxidase in a photocured poly(HEMA) film. / Keow, Ching Mai; Bakar, Fatimah Abu; Salleh, Abu Bakar; Lee, Yook Heng; Wagiran, Rahman; Siddiquee, Shafiquzzaman.

In: International Journal of Electrochemical Science, Vol. 7, No. 5, 2012, p. 4702-4715.

Research output: Contribution to journalArticle

Keow, Ching Mai ; Bakar, Fatimah Abu ; Salleh, Abu Bakar ; Lee, Yook Heng ; Wagiran, Rahman ; Siddiquee, Shafiquzzaman. / Screen-printed histamine biosensors fabricated from the entrapment of diamine oxidase in a photocured poly(HEMA) film. In: International Journal of Electrochemical Science. 2012 ; Vol. 7, No. 5. pp. 4702-4715.
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