Screen-printed biosensor with alkaline phosphatase immobilized in sol-gel/chitosan film for the detection of 2,4-dichlorophenoxyacetic acid

Kee Shyuan Loh, Yook Heng Lee, Musa Ahmad, Salmah Abdul Aziz, Zamri Ishak

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

An electrochemical biosensor for the detection of dichlorophenoxyacetic acid (2,4-D) based on a screen-printed carbon paste electrode (SPE) was developed. The biosensor film deposited on the SPE was a sol-gel/chitosan film containing immobilized alkaline phophatase enzyme (ALP). The 2,4-D herbicide was determined by the quantitative inhibition on the enzyme alkaline phosphatase. Ascorbic acid 2-phosphate (AA2P) was used as a substrate to indicate the inhibition by 2,4-D. The enzymic reaction with the substrate was monitored amperometrically at a potential of +600 mV and at optimum pH of 8.5. The linear response range towards the substrate AA2P was 10-80 μM. For 2,4-D determination, the maximum inhibition of the alkaline phosphatase was achieved after 80 ppb (60% inhibition) 2,4-D with a linear response range of 2-80 ppb.

Original languageEnglish
Pages (from-to)17-21
Number of pages5
JournalSensor Letters
Volume4
Issue number1
DOIs
Publication statusPublished - 2006

Fingerprint

Enzyme inhibition
phosphatases
Phosphatases
Chitosan
bioinstrumentation
Biosensors
Sol-gels
Ascorbic acid
gels
acids
Acids
ascorbic acid
Phosphates
Substrates
Enzymes
enzymes
phosphates
Electrodes
Carbon
Herbicides

Keywords

  • 2,4-dichlorophenoxyacetic acid
  • Disposable biosensor
  • Herbicide
  • Sol-gel/chitosan
  • Toxicity

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Signal Processing
  • Control and Systems Engineering

Cite this

Screen-printed biosensor with alkaline phosphatase immobilized in sol-gel/chitosan film for the detection of 2,4-dichlorophenoxyacetic acid. / Loh, Kee Shyuan; Lee, Yook Heng; Ahmad, Musa; Aziz, Salmah Abdul; Ishak, Zamri.

In: Sensor Letters, Vol. 4, No. 1, 2006, p. 17-21.

Research output: Contribution to journalArticle

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AU - Ishak, Zamri

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