Scoparia dulcis (SDF7) endowed with glucose uptake properties on L6 myotubes compared insulin

Joo Ee Beh, Jalifah Latip, Mohd Puad Abdullah, Amin Ismail, Muhajir Hamid

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Aim of the study: Insulin stimulates glucose uptake and promotes the translocation of glucose transporter 4 (Glut 4) to the plasma membrane on L6 myotubes. The aim of this study is to investigate affect of Scoparia dulcis Linn water extracts on glucose uptake activity and the Glut 4 translocation components (i.e., IRS-1, PI 3-kinase, PKB/Akt2, PKC and TC 10) in L6 myotubes compared to insulin. Materials and methods: Extract from TLC fraction-7 (SDF7) was used in this study. The L6 myotubes were treated by various concentrations of SDF7 (1 to 50μg/ml) and insulin (1 to 100 nM). The glucose uptake activities of L6 myotubes were evaluated using 2-Deoxy-D-glucose uptake assay in with or without fatty acid-induced medium. The Glut 4 translocation components in SDF7-treated L6 myotubes were detected using immunoblotting and quantified by densitometry compared to insulin. Plasma membrane lawn assay and glycogen colorimetry assay were carried out in SDF7- and insulin-treated L6 myotubes in this study. Results: Here, our data clearly shows that SDF7 possesses glucose uptake properties on L6 myotubes that are dose-dependent, time-dependent and plasma membrane Glut 4 expression-dependent. SDF7 successfully stimulates glucose uptake activity as potent as insulin at a maximum concentration of 50μg/ml at 480. min on L6 myotubes. Furthermore, SDF7 stimulates increased Glut 4 expression and translocation to plasma membranes at equivalent times. Even in the insulin resistance stage (free fatty acids-induced), SDF7-treated L6 myotubes were found to be more capable at glucose transport than insulin treatment. Conclusions: Thus, we suggested that Scoparia dulcis has the potential to be categorized as a hypoglycemic medicinal plant based on its good glucose transport properties.

Original languageEnglish
Pages (from-to)23-33
Number of pages11
JournalJournal of Ethnopharmacology
Volume129
Issue number1
DOIs
Publication statusPublished - May 2010

Fingerprint

Scoparia
Skeletal Muscle Fibers
Insulin
Facilitative Glucose Transport Proteins
Glucose
Cell Membrane
Colorimetry
Membrane Transport Proteins
Densitometry
Deoxyglucose
Medicinal Plants
Glycogen
Phosphatidylinositol 3-Kinases
Nonesterified Fatty Acids
Immunoblotting
Hypoglycemic Agents
Insulin Resistance
Fatty Acids

Keywords

  • Glucose transport
  • Glut 4
  • Insulin
  • Insulin resistance
  • Scoparia dulcis

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Scoparia dulcis (SDF7) endowed with glucose uptake properties on L6 myotubes compared insulin. / Beh, Joo Ee; Latip, Jalifah; Abdullah, Mohd Puad; Ismail, Amin; Hamid, Muhajir.

In: Journal of Ethnopharmacology, Vol. 129, No. 1, 05.2010, p. 23-33.

Research output: Contribution to journalArticle

Beh, Joo Ee ; Latip, Jalifah ; Abdullah, Mohd Puad ; Ismail, Amin ; Hamid, Muhajir. / Scoparia dulcis (SDF7) endowed with glucose uptake properties on L6 myotubes compared insulin. In: Journal of Ethnopharmacology. 2010 ; Vol. 129, No. 1. pp. 23-33.
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