Sall4 interacts with Nanog and co-occupies Nanog genomic sites in embryonic stem cells

Qiang Wu, Xi Chen, Jinqiu Zhang, Yuin Han Loh, Low Teck Yew, Weiwei Zhang, Wensheng Zhang, Siu Kwan Sze, Bing Lim, Huck Hui Ng

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

Embryonic stem (ES) cells are pluripotent cells with self-renewing property. Nanog is a homeobox transcription factor required to maintain ES cells in a non-differentiated state. Using affinity purification coupled to liquid chromatography-tandem mass spectrometry analysis, we identified Sall4 as a Nanog co-purified protein. Co-immunoprecipitation and glutathione S-transferase pulldown experiments confirmed the interaction between Nanog and Sall4. We showed that Nanog and Sall4 co-occupied Nanog and Sall4 enhancer regions in living ES cells. Knockdown of Nanog or Sall4 by RNA interference led to a reduction in Nanog and Sall4 enhancer activities, providing evidence that these factors are positively regulating these enhancers. Importantly, co-transfection of Sall4 with these ES cell-specific enhancers led to transactivation in heterologous somatic cells. Chromatin immunoprecipitation experiments also showed that Sall4 co-occupied many Nanog binding sites in ES cells. Our data implicate Sall4 as an important component of the transcription regulatory networks in ES cells by cooperating with Nanog. We suggest that Sall4 and Nanog form a regulatory circuit similar to that of Oct4 and Sox2. This study highlights the extensive regulatory loops connecting genes, which encode for key transcription factors in ES cells.

Original languageEnglish
Pages (from-to)24090-24094
Number of pages5
JournalJournal of Biological Chemistry
Volume281
Issue number34
DOIs
Publication statusPublished - 25 Aug 2006
Externally publishedYes

Fingerprint

Embryonic Stem Cells
Stem cells
Transcription Factors
Homeobox Genes
Chromatin Immunoprecipitation
Liquid chromatography
Transcription
Tandem Mass Spectrometry
RNA Interference
Glutathione Transferase
Immunoprecipitation
Liquid Chromatography
Transcriptional Activation
Chromatin
Purification
Transfection
Mass spectrometry
Genes
Experiments
Binding Sites

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sall4 interacts with Nanog and co-occupies Nanog genomic sites in embryonic stem cells. / Wu, Qiang; Chen, Xi; Zhang, Jinqiu; Loh, Yuin Han; Teck Yew, Low; Zhang, Weiwei; Zhang, Wensheng; Sze, Siu Kwan; Lim, Bing; Ng, Huck Hui.

In: Journal of Biological Chemistry, Vol. 281, No. 34, 25.08.2006, p. 24090-24094.

Research output: Contribution to journalArticle

Wu, Q, Chen, X, Zhang, J, Loh, YH, Teck Yew, L, Zhang, W, Zhang, W, Sze, SK, Lim, B & Ng, HH 2006, 'Sall4 interacts with Nanog and co-occupies Nanog genomic sites in embryonic stem cells', Journal of Biological Chemistry, vol. 281, no. 34, pp. 24090-24094. https://doi.org/10.1074/jbc.C600122200
Wu, Qiang ; Chen, Xi ; Zhang, Jinqiu ; Loh, Yuin Han ; Teck Yew, Low ; Zhang, Weiwei ; Zhang, Wensheng ; Sze, Siu Kwan ; Lim, Bing ; Ng, Huck Hui. / Sall4 interacts with Nanog and co-occupies Nanog genomic sites in embryonic stem cells. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 34. pp. 24090-24094.
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