Saccharification of polysaccharide content of palm kernel cake using enzymatic catalysis for production of biobutanol in acetone-butanol-ethanol fermentation

Hafiza Shukor, Peyman Abdeshahian, Najeeb Kaid Nasser Al-Shorgani, Aidil Abdul Hamid, Norliza Abd Rahman, Mohd. Sahaid Kalil

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In this work, hydrolysis of cellulose and hemicellulose content of palm kernel cake (PKC) by different types of hydrolytic enzymes was studied to evaluate monomeric sugars released for production of biobutanol by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) in acetone-butanol-ethanol (ABE) fermentation. Experimental results revealed that when PKC was hydrolyzed by mixed β-glucosidase, cellulase and mannanase, a total simple sugars of 87.81 ± 4.78 g/L were produced, which resulted in 3.75 ± 0.18 g/L butanol and 6.44 ± 0.43 g/L ABE at 168 h fermentation. In order to increase saccharolytic efficiency of enzymatic treatment, PKC was pretreated by liquid hot water before performing enzymatic hydrolysis. Test results showed that total reducing sugars were enhanced to 97.81 ± 1.29 g/L with elevated production of butanol and ABE up to 4.15 ± 1.18 and 7.12 ± 2.06 g/L, respectively which represented an A:B:E ratio of 7:11:1.

Original languageEnglish
Pages (from-to)206-213
Number of pages8
JournalBioresource Technology
Volume202
DOIs
Publication statusPublished - 1 Feb 2016

Fingerprint

Saccharification
Butanols
catalysis
Polysaccharides
Acetone
acetone
Butenes
polysaccharide
Fermentation
Catalysis
fermentation
ethanol
sugar
Ethanol
Sugars
hydrolysis
cellulose
Glucosidases
Clostridium
Enzymatic hydrolysis

Keywords

  • Butanol production
  • Clostridium saccharoperbutylacetonicum N1-4
  • Enzyme treatment
  • Fermentable sugars
  • Palm kernel cake

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Saccharification of polysaccharide content of palm kernel cake using enzymatic catalysis for production of biobutanol in acetone-butanol-ethanol fermentation. / Shukor, Hafiza; Abdeshahian, Peyman; Al-Shorgani, Najeeb Kaid Nasser; Abdul Hamid, Aidil; Abd Rahman, Norliza; Kalil, Mohd. Sahaid.

In: Bioresource Technology, Vol. 202, 01.02.2016, p. 206-213.

Research output: Contribution to journalArticle

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